The use of the D-, L- aspartic ratio in decalcified collagen from human dentin as an estimator of human age
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11569570
Knihovny.cz E-resources
- MeSH
- Chromatography, Gas MeSH
- Dentin chemistry MeSH
- Adult MeSH
- Hydrolysis MeSH
- Isomerism MeSH
- Calcinosis MeSH
- Collagen analysis metabolism MeSH
- Aspartic Acid analysis chemistry MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Forensic Medicine methods MeSH
- Age Determination by Teeth methods MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Collagen MeSH
- Aspartic Acid MeSH
Among the methods dealing with the age estimation, the evaluation of the ratio of the D-, L- form of the aspartic acid in tissues with a low metabolic turnover is considered to be the most precise. We introduced demineralization of the dentin with 0.5 M EDTA adjusted to pH = 7.4. The advantage of such a procedure is that after demineralization we obtained pure insoluble protein (collagen) and soluble noncollagenous proteins in one step. In this study we analyzed insoluble collagen. The amino acids obtained after the hydrolysis were derivatized into TFA isopropyl esters and analyzed by gas chromatography on Chirasil L-Val capillary column. We analyzed human dentin from the lower canines. The correlation coefficient was 0.93 for our set of 71 persons. The result concurred with those of other scientists.
Characterization of Two Historic Smallpox Specimens from a Czech Museum
Changes in colour of different human tissues as a marker of age
