The use of the D-, L- aspartic ratio in decalcified collagen from human dentin as an estimator of human age

. 2001 Sep ; 46 (5) : 1228-31.

Jazyk angličtina Země Spojené státy americké Médium print

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid11569570

Among the methods dealing with the age estimation, the evaluation of the ratio of the D-, L- form of the aspartic acid in tissues with a low metabolic turnover is considered to be the most precise. We introduced demineralization of the dentin with 0.5 M EDTA adjusted to pH = 7.4. The advantage of such a procedure is that after demineralization we obtained pure insoluble protein (collagen) and soluble noncollagenous proteins in one step. In this study we analyzed insoluble collagen. The amino acids obtained after the hydrolysis were derivatized into TFA isopropyl esters and analyzed by gas chromatography on Chirasil L-Val capillary column. We analyzed human dentin from the lower canines. The correlation coefficient was 0.93 for our set of 71 persons. The result concurred with those of other scientists.

Nejnovějších 20 citací...

Zobrazit více v
Medvik | PubMed

Characterization of Two Historic Smallpox Specimens from a Czech Museum

. 2017 Jul 27 ; 9 (8) : . [epub] 20170727

Changes in colour of different human tissues as a marker of age

. 2007 Mar ; 121 (2) : 158-62. [epub] 20061118

Najít záznam

Citační ukazatele

Nahrávání dat ...

Možnosti archivace

Nahrávání dat ...