A suite of spin-state-selective excitation (S3E) NMR experiments for the measurements of small one-bond (13C-13C, 15N-13C) and two-bond (1H-13C, 1H-15N) coupling constants in 13C,15N labeled purine and pyrimidine bases is presented. The incorporation of band-selective shaped pulses, elimination of the cross talk between alpha and beta sub-spectra, and accuracy and precision of the proposed approach are discussed. Merits of using S3E rather than alpha/beta-half-filter are demonstrated using results obtained on isotopically labeled DNA oligonucleotides.

Masaryk University Faculty of Science National Centre for Biomolecular Research Brno Czech Republic

Zobrazit více v PubMed

J Biomol NMR. 1997 Jul;10(1):89-94 PubMed

Biochemistry. 1992 Sep 22;31(37):8898-905 PubMed

J Biomol NMR. 1999 Dec;15(4):335-8 PubMed

J Magn Reson. 1999 Jun;138(2):334-42 PubMed

J Biomol NMR. 1992 Nov;2(6):661-5 PubMed

J Magn Reson. 1997 Feb;124(2):512-5 PubMed

Nat Struct Biol. 1998 Dec;5(12):1065-74 PubMed

J Biomol NMR. 1998 Oct;12(3):435-41 PubMed

J Biomol NMR. 2000 Mar;16(3):221-7 PubMed

Nat Struct Biol. 1997 Sep;4(9):732-8 PubMed

J Magn Reson. 1999 Mar;137(1):237-42 PubMed

J Magn Reson. 1999 Sep;140(1):32-40 PubMed

Science. 1997 Nov 7;278(5340):1111-4 PubMed

S3EPY: a Sparky extension for determination of small scalar couplings from spin-state-selective excitation NMR experiments

13C-detected NMR experiments for measuring chemical shifts and coupling constants in nucleic acid bases

Experiments for correlating quaternary carbons in RNA bases