Silver electrode as a sensor for determination of zinc in cell cultivation medium
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11811961
DOI
10.1006/abio.2001.5484
PII: S0003269701954843
Knihovny.cz E-resources
- MeSH
- Genes, myb MeSH
- Ion-Selective Electrodes * MeSH
- Culture Media chemistry metabolism MeSH
- Chickens MeSH
- Metallothionein genetics MeSH
- Oncogene Protein p65(gag-jun) biosynthesis genetics MeSH
- Promoter Regions, Genetic MeSH
- Silver * MeSH
- Transformation, Genetic MeSH
- Cell Line, Transformed MeSH
- Zinc analysis MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Culture Media MeSH
- Metallothionein MeSH
- Oncogene Protein p65(gag-jun) MeSH
- Silver * MeSH
- Zinc MeSH
Use of the silver electrode as a sensor for the monitoring of zinc in cell growth medium is described. Zinc at silver electrodes provides specific voltammetric signal, which is affected by solution components. Signals of zinc ions in phosphate buffer solutions with and without cell growth medium were compared. Common DMEM cell culture medium was used for the cultivation of a cell line of v-myb-transformed chicken monoblasts and its variants expressing v-jun and c-jun in a zinc-dependent manner. Electrochemical results showed zinc concentrations in the medium coincide very well with the jun expression. With respect to the low toxicity of silver for eukaryotic cells, silver electrodes represent promising tools for the determination of zinc concentrations in vivo without the potential risk of a cell culture damage.
References provided by Crossref.org
Amperometric Sensor for Detection of Chloride Ions
