Silver electrode as a sensor for determination of zinc in cell cultivation medium
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
11811961
DOI
10.1006/abio.2001.5484
PII: S0003269701954843
Knihovny.cz E-zdroje
- MeSH
- geny myb MeSH
- iontově selektivní elektrody * MeSH
- kultivační média chemie metabolismus MeSH
- kur domácí MeSH
- metalothionein genetika MeSH
- onkogenní protein p65(gag-jun) biosyntéza genetika MeSH
- promotorové oblasti (genetika) MeSH
- stříbro * MeSH
- transformace genetická MeSH
- transformované buněčné linie MeSH
- zinek analýza MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- kultivační média MeSH
- metalothionein MeSH
- onkogenní protein p65(gag-jun) MeSH
- stříbro * MeSH
- zinek MeSH
Use of the silver electrode as a sensor for the monitoring of zinc in cell growth medium is described. Zinc at silver electrodes provides specific voltammetric signal, which is affected by solution components. Signals of zinc ions in phosphate buffer solutions with and without cell growth medium were compared. Common DMEM cell culture medium was used for the cultivation of a cell line of v-myb-transformed chicken monoblasts and its variants expressing v-jun and c-jun in a zinc-dependent manner. Electrochemical results showed zinc concentrations in the medium coincide very well with the jun expression. With respect to the low toxicity of silver for eukaryotic cells, silver electrodes represent promising tools for the determination of zinc concentrations in vivo without the potential risk of a cell culture damage.
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