A biosensor for the detection of triazine and phenylurea herbicides designed using Photosystem II coupled to a screen-printed electrode
Language English Country United States Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
11857287
DOI
10.1002/bit.10190
PII: 10.1002/bit.10190
Knihovny.cz E-resources
- MeSH
- Biosensing Techniques instrumentation methods MeSH
- Chromatography, Liquid instrumentation methods MeSH
- Electrodes MeSH
- Phenylurea Compounds analysis MeSH
- Photosynthetic Reaction Center Complex Proteins metabolism MeSH
- Photosystem II Protein Complex MeSH
- Herbicides analysis MeSH
- Cyanobacteria chemistry physiology MeSH
- Triazines * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Phenylurea Compounds MeSH
- Photosynthetic Reaction Center Complex Proteins MeSH
- Photosystem II Protein Complex MeSH
- Herbicides MeSH
- Triazines * MeSH
A biosensor for the detection of triazine- and phenylurea-type herbicides was constructed using isolated Photosystem II (PS II) complexes as a biosensing element. PSII isolated from the thermophilic cyanobacterium Synechococcus elongatus was immobilized on the surface of a screen-printed sensor composed of a graphite working electrode and Ag/AgCl reference electrode deposited on a polymeric substrate. The biosensor was mounted in a flow microcell with illumination. The principle of the detection was based on the fact that herbicides selectively block PSII electron transport activity in a concentration-dependent manner. Changes of the activity were registered amperometrically as the rate of photoreduction of an artificial electron acceptor. The setup resulted in a reusable herbicide biosensor with a good stability (half-life of 24 h) and limit of detection of approximately 10(-9) M for diuron, atrazine and simazine.
References provided by Crossref.org
Photosystem II in bio-photovoltaic devices
Fast Ecotoxicity Detection Using Biosensors
