An origin of DNA replication from streptomycete phage phi U1
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11898336
DOI
10.1007/bf02817990
Knihovny.cz E-resources
- MeSH
- Bacteriophages genetics MeSH
- DNA, Viral physiology MeSH
- Molecular Sequence Data MeSH
- DNA Replication * MeSH
- Virus Replication MeSH
- Replication Origin * MeSH
- Base Sequence MeSH
- Streptomyces virology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Viral MeSH
A DNA fragment from phage phi U1 containing an origin of DNA replication was identified. This fragment, designated ori, was able to support the maintenance in Streptomyces lividans of a plasmid lacking a functional Gram-positive ori. The sequence of the minimal ori fragment was determined and analyzed. The minimal fragment conferring replication origin function contained a number of direct and inverted repeats. The absence of an open reading frame in this ori fragment indicates that host factors alone were sufficient to initiate replication at ori.
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