Growth-associated production of poly-3-hydroxybutyrate by Bacillus mycoides
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11898337
DOI
10.1007/bf02817991
Knihovny.cz E-resources
- MeSH
- Bacillus growth & development metabolism MeSH
- Hydroxybutyrates chemistry metabolism MeSH
- Kinetics MeSH
- Culture Media MeSH
- Molecular Weight MeSH
- Polyesters chemistry metabolism MeSH
- Sucrose MeSH
- Carbon analysis MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Hydroxybutyrates MeSH
- Culture Media MeSH
- poly-beta-hydroxybutyrate MeSH Browser
- Polyesters MeSH
- Sucrose MeSH
- Carbon MeSH
Bacillus mycoides strain RIJ B-017, a growth-associated poly-3-hydroxybutyrate (PHB) producer was grown on sucrose-containing media. PHB accumulated in cells up to 72% of dry cell mass. The overall maximum value of PHB yield (Yp/s) and productivities (Qp and qp) 250 mgp/gs, 120 mgp L-1 h-1 and 30 mgp gx-1 h-1, respectively, were obtained at 15 g/L sucrose. Differential scanning calorimeter heating curve showed two peaks, one at 95.9 degrees C and another at 165.4 degrees C with a shoulder around 154.6 degrees C. The viscosity-average molar mass in chloroform at 27 degrees C was 505 kDa. The carbon content of PHB was 55.4% of the mass.
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