Activation of macrophages by gliadin fragments: isolation and characterization of active peptide
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11927649
Knihovny.cz E-resources
- MeSH
- Macrophage Activation drug effects MeSH
- Chemokine CCL5 biosynthesis MeSH
- Gliadin pharmacology MeSH
- Interleukin-10 biosynthesis MeSH
- Mice, Inbred C3H MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Nitric Oxide biosynthesis MeSH
- Peptide Fragments isolation & purification pharmacology MeSH
- Nitric Oxide Synthase Type II MeSH
- Nitric Oxide Synthase metabolism MeSH
- Tumor Necrosis Factor-alpha biosynthesis MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Chemokine CCL5 MeSH
- Gliadin MeSH
- Interleukin-10 MeSH
- Nos2 protein, mouse MeSH Browser
- Nitric Oxide MeSH
- Peptide Fragments MeSH
- Nitric Oxide Synthase Type II MeSH
- Nitric Oxide Synthase MeSH
- Tumor Necrosis Factor-alpha MeSH
Celiac disease, induced by dietary gluten, is characterized by mucosal atrophy and local inflammation associated with cell infiltration and activation. Unlike other food proteins, gluten and its proteolytic fragments, besides inducing a specific immune response, were shown to activate components of innate immunity and cause, e.g., direct stimulation of TNF-alpha and IL-10 and a significant rise in NO production by peritoneal macrophages. The identity of the active fragments was established by separating the peptic digest of gliadin by RP-HPLC chromatography. The purest fraction with the highest activity was analyzed by mass spectrometry, and the gliadin peptide sequence was identified as VSFQQPQQQYPSSQ. This peptide (T) and its N- and C-terminally shortened forms (A, B, C and D, E, F) were synthesized. Peptide B (FQQPQQQYPSSQ) elicited the highest TNF-alpha, IL-10, and RANTES secretion and increase in IFN-gamma-primed NO production by mouse macrophages. In contrast, C-terminally shortened peptides had a lower ability to stimulate macrophages than the native form.
Gliadin fragments promote migration of dendritic cells
Gliadin peptides activate blood monocytes from patients with celiac disease
