Lipopolysaccharide induces inflammatory cytokines in the pig amnion
Language English Country Netherlands Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
12052338
DOI
10.1016/s0165-2427(02)00025-9
PII: S0165242702000259
Knihovny.cz E-resources
- MeSH
- Amnion immunology metabolism pathology MeSH
- Chorioamnionitis immunology metabolism pathology MeSH
- Immunohistochemistry veterinary MeSH
- Interleukin-8 biosynthesis MeSH
- Lipopolysaccharides immunology pharmacology MeSH
- RNA, Messenger chemistry genetics MeSH
- Swine, Miniature MeSH
- Tumor Cells, Cultured MeSH
- Swine Diseases immunology metabolism MeSH
- Fetus immunology MeSH
- Reverse Transcriptase Polymerase Chain Reaction veterinary MeSH
- Swine MeSH
- Pregnancy MeSH
- Tumor Necrosis Factor-alpha biosynthesis MeSH
- Transforming Growth Factor beta biosynthesis MeSH
- Transforming Growth Factor beta1 MeSH
- Animals MeSH
- Check Tag
- Pregnancy MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Interleukin-8 MeSH
- Lipopolysaccharides MeSH
- RNA, Messenger MeSH
- Tumor Necrosis Factor-alpha MeSH
- Transforming Growth Factor beta MeSH
- Transforming Growth Factor beta1 MeSH
Inflammatory mediators that are induced by gram-negative bacteria in the course of intrauterine infections threaten successful pregnancy. To compare the effect of two different routes of cytokine induction, bacterial lipopolysaccharide (LPS) was administered in vivo either into the cord vein or into the amniotic cavity of pig fetuses in the second half of gestation for 20 h and cytokines were detected in the amnion.Tumor necrosis factor-alpha (TNF-alpha) and interleukin-8 (IL-8) were induced in the amniotic epithelium after intra-amniotic but not after intra-venous administration of LPS. The presence of IL-8 was confirmed by RT-PCR. In contrast, transforming growth factor-beta1 (TGF-beta1) was expressed constitutively and was found in all samples of the amniotic epithelium. Amniotic fluid contained only minute levels of TNF-alpha. IL-8 levels in amniotic fluid increased after the treatment with LPS and the highest IL-8 levels were found in dead LPS-treated fetuses.
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