Expression of inflammatory markers in pig amnion after intraamniotic infection with nonpathogenic or enteropathogenic Escherichia coli
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
15881414
DOI
10.1007/bf02931560
Knihovny.cz E-zdroje
- MeSH
- amnion imunologie mikrobiologie MeSH
- biologické markery metabolismus MeSH
- cytokiny metabolismus MeSH
- Escherichia coli imunologie patogenita MeSH
- infekce vyvolané Escherichia coli imunologie mikrobiologie veterinární MeSH
- infekční komplikace v těhotenství imunologie mikrobiologie veterinární MeSH
- interferon gama metabolismus MeSH
- interleukin-1 metabolismus MeSH
- interleukin-10 metabolismus MeSH
- nemoci prasat imunologie mikrobiologie MeSH
- prasata MeSH
- těhotenství MeSH
- TNF-alfa metabolismus MeSH
- zánět imunologie MeSH
- zvířata MeSH
- Check Tag
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- biologické markery MeSH
- cytokiny MeSH
- interferon gama MeSH
- interleukin-1 MeSH
- interleukin-10 MeSH
- TNF-alfa MeSH
The pig amnion was in vivo intraamniotically infected with E. coli for 10 h at 80-85 d of gestation either with the nonpathogenic O86 strain or enteropathogenic O55 strain. TNF-alpha, IL-10, IL-1beta and IFN-gamma were determined in amniotic fluids by ELISA, the expression of cytokines and some other inflammatory markers was determined by immunohistochemistry. Intraamniotic infection induced high levels of TNF-alpha in amniotic fluids which correlated with bacterial virulence whereas IL-10 was induced only by O86. The IL-1beta level did not increase significantly and was expressed in all infected membranes. IFN-gamma was negligible or absent. TNF-alpha, IL-12p40, calprotectin, HSP65 and gp91phox were found by immunohistochemistry only in amnion membranes infected with the enteropathogenic strain 055.
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