Expression of inflammatory markers in pig amnion after intraamniotic infection with nonpathogenic or enteropathogenic Escherichia coli
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15881414
DOI
10.1007/bf02931560
Knihovny.cz E-resources
- MeSH
- Amnion immunology microbiology MeSH
- Biomarkers metabolism MeSH
- Cytokines metabolism MeSH
- Escherichia coli immunology pathogenicity MeSH
- Escherichia coli Infections immunology microbiology veterinary MeSH
- Pregnancy Complications, Infectious immunology microbiology veterinary MeSH
- Interferon-gamma metabolism MeSH
- Interleukin-1 metabolism MeSH
- Interleukin-10 metabolism MeSH
- Swine Diseases immunology microbiology MeSH
- Swine MeSH
- Pregnancy MeSH
- Tumor Necrosis Factor-alpha metabolism MeSH
- Inflammation immunology MeSH
- Animals MeSH
- Check Tag
- Pregnancy MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biomarkers MeSH
- Cytokines MeSH
- Interferon-gamma MeSH
- Interleukin-1 MeSH
- Interleukin-10 MeSH
- Tumor Necrosis Factor-alpha MeSH
The pig amnion was in vivo intraamniotically infected with E. coli for 10 h at 80-85 d of gestation either with the nonpathogenic O86 strain or enteropathogenic O55 strain. TNF-alpha, IL-10, IL-1beta and IFN-gamma were determined in amniotic fluids by ELISA, the expression of cytokines and some other inflammatory markers was determined by immunohistochemistry. Intraamniotic infection induced high levels of TNF-alpha in amniotic fluids which correlated with bacterial virulence whereas IL-10 was induced only by O86. The IL-1beta level did not increase significantly and was expressed in all infected membranes. IFN-gamma was negligible or absent. TNF-alpha, IL-12p40, calprotectin, HSP65 and gp91phox were found by immunohistochemistry only in amnion membranes infected with the enteropathogenic strain 055.
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