Escherichia coli administered into pig amniotic cavity appear in fetal airways and attract macrophages into fetal lungs
Language English Country Czech Republic Media print
Document type Journal Article
PubMed
12470205
Knihovny.cz E-resources
- MeSH
- Macrophages, Alveolar immunology microbiology ultrastructure MeSH
- Chorioamnionitis immunology microbiology MeSH
- Microscopy, Electron MeSH
- Escherichia coli growth & development pathogenicity MeSH
- Escherichia coli Infections immunology MeSH
- Swine, Miniature MeSH
- Disease Models, Animal MeSH
- Lung embryology immunology microbiology MeSH
- Fetus immunology microbiology MeSH
- Amniotic Fluid immunology microbiology MeSH
- Pregnancy MeSH
- Virulence MeSH
- Animals MeSH
- Check Tag
- Pregnancy MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Escherichia coli (2 x 10(4) bacteria) of the non-pathogenic O86 strain or enteropathogenic O55 strain were administered into the pig amniotic cavity at 79 to 86 days of gestation for six or ten hours. Translocation of bacteria into fetal lungs was confirmed by cultivation as well as by light and electron microscopy. Infection caused an influx of macrophages that were immunostained in cryostat sections by monoclonal antibody recognizing calprotectin.
High Mobility Group Box 1 in Pig Amniotic Membrane Experimentally Infected with E. coli O55
