Genetic variation in the promoter region of the basic fibroblast growth factor gene
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
12590983
DOI
10.1016/s0198-8859(02)00820-0
PII: S0198885902008200
Knihovny.cz E-zdroje
- MeSH
- 5' přiléhající oblast DNA MeSH
- bodová mutace MeSH
- fibroblastový růstový faktor 2 genetika MeSH
- genetická variace * MeSH
- lidé středního věku MeSH
- lidé MeSH
- promotorové oblasti (genetika) * MeSH
- senioři MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fibroblastový růstový faktor 2 MeSH
Basic fibroblast growth factor (bFGF) is a member of the fibroblast growth factor family that possess broad mitogenic and cell survival activities and is involved in a variety of biological processes. We investigated possible genetic polymorphism in the promoter and 5' flanking region of the bFGF gene. Polymorphism was analysed by means of heteroduplex analysis, fragments with altered mobility were sequenced. Three novel substitutions (-553T/A, -834T/A and -921C/G) were identified in the promoter region. Allele frequencies in a sample of healthy Caucasian subjects (n=126) were determined by polymerase chain reactions followed by restriction analyses with specific endonucleases. The frequencies of the mutated alleles (-553A, -834A and -921G) were 0.04, 0.05 and 0.14, respectively. Newly identified variants in the bFGF gene promoter appear to be common polymorphisms in the Czech population.
Citace poskytuje Crossref.org
