Genetic variation in the promoter region of the basic fibroblast growth factor gene
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12590983
DOI
10.1016/s0198-8859(02)00820-0
PII: S0198885902008200
Knihovny.cz E-resources
- MeSH
- 5' Flanking Region MeSH
- Point Mutation MeSH
- Fibroblast Growth Factor 2 genetics MeSH
- Genetic Variation * MeSH
- Middle Aged MeSH
- Humans MeSH
- Promoter Regions, Genetic * MeSH
- Aged MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Fibroblast Growth Factor 2 MeSH
Basic fibroblast growth factor (bFGF) is a member of the fibroblast growth factor family that possess broad mitogenic and cell survival activities and is involved in a variety of biological processes. We investigated possible genetic polymorphism in the promoter and 5' flanking region of the bFGF gene. Polymorphism was analysed by means of heteroduplex analysis, fragments with altered mobility were sequenced. Three novel substitutions (-553T/A, -834T/A and -921C/G) were identified in the promoter region. Allele frequencies in a sample of healthy Caucasian subjects (n=126) were determined by polymerase chain reactions followed by restriction analyses with specific endonucleases. The frequencies of the mutated alleles (-553A, -834A and -921G) were 0.04, 0.05 and 0.14, respectively. Newly identified variants in the bFGF gene promoter appear to be common polymorphisms in the Czech population.
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