In vitro interaction of macrocyclic photosensitizers with intact mitochondria: a spectroscopic study
Language English Country Netherlands Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
12595077
DOI
10.1016/s0304-4165(02)00511-1
PII: S0304416502005111
Knihovny.cz E-resources
- MeSH
- Algorithms MeSH
- Photochemotherapy MeSH
- Photosensitizing Agents chemical synthesis chemistry MeSH
- Mitochondria, Liver chemistry MeSH
- Molecular Structure MeSH
- Porphyrins chemical synthesis chemistry MeSH
- Swine MeSH
- Spectrum Analysis, Raman MeSH
- Spectrophotometry MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Photosensitizing Agents MeSH
- Porphyrins MeSH
Six water-soluble macrocyclic photosensitizers, the members of two groups of expanded porphyrins (metallotexaphyrins and free-base sapphyrins) containing hydrophilic substituents and meso-tetra(4-sulfonatophenyl)-porphyrin, were tested by UV-Vis absorption and resonance Raman spectroscopy in the in vitro binding experiments with intact mitochondria isolated from swine liver. Studied macrocycles showed markedly different affinity to mitochondria. The highest uptake was observed for sapphyrin-sugar conjugate and metallotexaphyrins. Sapphyrin-polyamine conjugates exhibit something less affinity to mitochondria, while the porphyrin of anionic character showed very low mitochondrial uptake. Obtained spectroscopic results confirm that the binding process altered the self-aggregation degree of expanded porphyrins.
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