Detection of pap-, sfa- and afa-specific DNA sequences in Escherichia coli strains isolated from extraintestinal material
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12630326
DOI
10.1007/bf02818678
Knihovny.cz E-resources
- MeSH
- Adhesins, Escherichia coli genetics physiology MeSH
- Bacterial Adhesion physiology MeSH
- Fimbriae, Bacterial physiology MeSH
- Cystitis microbiology MeSH
- Child MeSH
- DNA, Bacterial chemistry genetics MeSH
- Adult MeSH
- Escherichia coli genetics isolation & purification pathogenicity MeSH
- Genital Diseases, Female microbiology MeSH
- Escherichia coli Infections microbiology MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Polymerase Chain Reaction MeSH
- Child, Preschool MeSH
- Pyelonephritis microbiology MeSH
- Virulence MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Child, Preschool MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adhesins, Escherichia coli MeSH
- DNA, Bacterial MeSH
P-fimbriae, S-fimbriae and AFA-adhesins are virulence factors responsible for adherence of Escherichia coli strains to extraintestinal host-cell surface. Detection of pap-, sfa- and afa-specific sequences performed by PCR revealed 74% pap+, 65% sfa+, and 8.3% afa+ strains in a group of 84 extraintestinal E. coli isolates. Detection in a group of fecal strains showed 29% pap+, 21% sfa+ and 4% afa+ strains. pap together with sfa were found as the most frequent combination (56%) among extraintestinal isolates probably due to localization of pap- and sfa-operons on a common pathogenicity island. The occurrence of afa-specific sequence among 56 urine strains was 11%, although no afa+ strain was detected among 28 gynecological isolates. No strains with detected adhesin operons were found among twenty (24%) extraintestinal E. coli strains.
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