Characterization of telomere-subtelomere junctions in Silene latifolia
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Chromatin chemistry genetics MeSH
- X Chromosome MeSH
- Chromosomes, Plant MeSH
- DNA, Plant genetics MeSH
- Genetic Variation MeSH
- In Situ Hybridization, Fluorescence MeSH
- Terminal Repeat Sequences genetics MeSH
- Molecular Sequence Data MeSH
- Nucleosomes chemistry genetics MeSH
- Base Sequence MeSH
- Sequence Homology, Nucleic Acid MeSH
- Silene genetics MeSH
- Tandem Repeat Sequences genetics MeSH
- Telomere genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Chromatin MeSH
- DNA, Plant MeSH
- Nucleosomes MeSH
Telomere-associated regions represent boundaries between the relatively homogeneous telomeres and the subtelomeres, which show much greater heterogeneity in chromatin structure and DNA composition. Although a major fraction of subtelomeres is usually formed by a limited number of highly repeated DNA sequence families, their mutual arrangement, attachment to telomeres and the presence of interspersed unique or low-copy-number sequences make these terminal domains chromosome specific. In this study, we describe the structures of junctions between telomeres and a major subtelomeric repeat of the plant Silene latifolia, X43.1. Our results show that on individual chromosome arms, X43.1 is attached to the telomere either directly at sites corresponding to nucleosome boundaries previously mapped in this sequence, or via other spacer sequences, both previously characterized and newly described ones. Sites of telomere junctions are non-random in all the telomere-associated sequences analysed. These data obtained at the molecular level have been verified using in situ hybridization to metaphase chromosomes and extended DNA fibres.
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