Specific in vitro cleavage of Mason-Pfizer monkey virus capsid protein: evidence for a potential role of retroviral protease in early stages of infection
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.
Grant support
RR 12878
NCRR NIH HHS - United States
RR 13948
NCRR NIH HHS - United States
PubMed
12781718
DOI
10.1016/s0042-6822(03)00128-4
PII: S0042682203001284
Knihovny.cz E-resources
- MeSH
- COS Cells MeSH
- Endopeptidases metabolism MeSH
- Gene Products, gag metabolism MeSH
- HIV-1 metabolism MeSH
- HIV Protease metabolism MeSH
- Mason-Pfizer monkey virus metabolism physiology MeSH
- Nucleocapsid chemistry metabolism MeSH
- Virus Replication * MeSH
- Amino Acid Sequence MeSH
- Capsid Proteins chemistry metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Names of Substances
- Endopeptidases MeSH
- Gene Products, gag MeSH
- HIV Protease MeSH
- Capsid Proteins MeSH
Processing of Gag polyproteins by viral protease (PR) leads to reorganization of immature retroviral particles and formation of a ribonucleoprotein core. In some retroviruses, such as HIV and RSV, cleavage of a spacer peptide separating capsid and nucleocapsid proteins is essential for the core formation. We show here that no similar spacer peptide is present in the capsid-nucleocapsid (CA-NC) region of Mason-Pfizer monkey virus (M-PMV) and that the CA protein is cleaved in vitro by the PR within the major homology region (MHR) and the NC protein in several sites at the N-terminus. The CA cleavage product was also identified shortly after penetration of M-PMV into COS cells, suggesting that the protease-catalyzed cleavage is involved in core disintegration.
References provided by Crossref.org
PF74 and Its Novel Derivatives Stabilize Hexameric Lattice of HIV-1 Mature-Like Particles
In vitro methods for testing antiviral drugs
Role of Mason-Pfizer monkey virus CA-NC spacer peptide-like domain in assembly of immature particles
HIV-1 protease-induced apoptosis
