The envelope glycoprotein (Env) plays a crucial role in the retroviral life cycle by mediating primary interactions with the host cell. As described previously and expanded on in this paper, Env mediates the trafficking of immature Mason-Pfizer monkey virus (M-PMV) particles to the plasma membrane (PM). Using a panel of labeled RabGTPases as endosomal markers, we identified Env mostly in Rab7a- and Rab9a-positive endosomes. Based on an analysis of the transport of recombinant fluorescently labeled M-PMV Gag and Env proteins, we propose a putative mechanism of the intracellular trafficking of M-PMV Env and immature particles. According to this model, a portion of Env is targeted from the trans-Golgi network (TGN) to Rab7a-positive endosomes. It is then transported to Rab9a-positive endosomes and back to the TGN. It is at the Rab9a vesicles where the immature particles may anchor to the membranes of the Env-containing vesicles, preventing Env recycling to the TGN. These Gag-associated vesicles are then transported to the plasma membrane.

Department of Biochemistry and Microbiology University of Chemistry and Technology 166 28 Prague Czech Republic

Department of Biotechnology University of Chemistry and Technology 166 28 Prague Czech Republic

Emory Vaccine Center at the Yerkes National Primate Research Center Emory University Atlanta GA 30329 USA

Imaging methods core facility at BIOCEV Faculty of Science Charles University 252 50 Prague Czech Republic

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Interaction Interface of Mason-Pfizer Monkey Virus Matrix and Envelope Proteins

The Current View of Retroviruses as Seen from the Shoulders of a Giant