The intracellular transport of Mason-Pfizer monkey virus (M-PMV) assembled capsids from the pericentriolar region to the plasma membrane (PM) requires trafficking of envelope glycoprotein (Env) to the assembly site via the recycling endosome. However, it is unclear if Env-containing vesicles play a direct role in trafficking capsids to the PM. Using live cell microscopy, we demonstrate, for the first time, anterograde co-transport of Gag and Env. Nocodazole disruption of microtubules had differential effects on Gag and Env trafficking, with pulse-chase assays showing a delayed release of Env-deficient virions. Particle tracking demonstrated an initial loss of linear movement of GFP-tagged capsids and mCherry-tagged Env, followed by renewed movement of Gag but not Env at 4h post-treatment. Thus, while delayed capsid trafficking can occur in the absence of microtubules, efficient anterograde transport of capsids appears to be mediated by microtubule-associated Env-containing vesicles.

Department of Biochemistry and Microbiology Institute of Chemical Technology Technicka 3 166 28 Prague Czech Republic

Department of Pediatrics Emory University School of Medicine 2015 Uppergate Drive Atlanta GA 30322 USA

Emory Vaccine Center Yerkes National Primate Research Center 954 Gatewood Road NE Atlanta GA 30329 USA

Laboratory of Persistent Viral Diseases Rocky Mountain Laboratories National Institute of Allergy and Infectious Diseases Hamilton MT USA

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Interaction Interface of Mason-Pfizer Monkey Virus Matrix and Envelope Proteins

Mason-Pfizer Monkey Virus Envelope Glycoprotein Cycling and Its Vesicular Co-Transport with Immature Particles

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