The Escherichia fergusonii iucABCD iutA genes are located within a larger chromosomal region similar to pathogenicity Islands
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.
Grant support
R01 DE1 2488
NIDCR NIH HHS - United States
R01 DE1 3759
NIDCR NIH HHS - United States
PubMed
12800494
DOI
10.1007/bf02930946
Knihovny.cz E-resources
- MeSH
- Chromosomes, Bacterial genetics MeSH
- Escherichia genetics metabolism pathogenicity MeSH
- Genomic Library MeSH
- Mutagenesis, Insertional MeSH
- Cosmids MeSH
- Hydroxamic Acids metabolism MeSH
- Chromosome Mapping * MeSH
- Molecular Sequence Data MeSH
- Bacterial Outer Membrane Proteins genetics metabolism MeSH
- Escherichia coli Proteins genetics metabolism MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Blotting, Southern MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Names of Substances
- aerobactin receptor MeSH Browser
- aerobactin MeSH Browser
- Hydroxamic Acids MeSH
- Bacterial Outer Membrane Proteins MeSH
- Escherichia coli Proteins MeSH
Three strains of Escherichia fergusonii (EF873, EF1496, EF939) of 50 strains tested produced the hydroxamate siderophore aerobactin. Screening of a cosmid library of the strain EF873 chromosomal DNA (in aerobactin nonproducing Escherichia coli VCS257) for aerobactin production identified iucABCD and iutA gene orthologues. The predicted IucABCD and IutA proteins showed 59-65% identity to the corresponding proteins of Shigella flexneri and E. coli. Aerobactin molecules synthesized by E. fergusonii and E. coli strains stimulated growth of aerobactin indicator strains harboring either E. coli or E. fergusonii iutA genes. In the 12 kb upstream and 17 kb downstream regions of the iuc and iut genes, 20 additional ORFs were identified. Their gene products showed homology to proteins from E. coli, S. flexneri, Klebsiella aerogenes, Pseudomonas aeruginosa and Vibrio cholerae. Probes recognizing DNA sequences from a region of more than 25 kb, which included the iucABCD and iutA genes, hybridized with chromosomal DNA of two aerobactin-producing strains (EF873 and EF939), but not with other nonproducing E. fergusonii strains tested. These data, together with the genetic organization of this region, suggest that E. fergusonii iucABCD iutA genes are a portion of a larger segment of DNA similar to pathogenicity islands of other bacteria.
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