Genetic polymorphism of 83 isolates of E. coli, derived from 4 species of artiodactyla animals living in a relatively close contact on the grounds of a theme park ZOO Safarii Swierkocin (Poland) was determined using the rep-PCR fingerprinting method, which utilizes oligonucleotide primers matching interspersed repetitive DNA sequences in PCR reaction to yield DNA fingerprints of individual bacterial isolates based on repetitive extragenic palindrome (REP) primers. The fingerprint patterns demonstrated the essential polymorphism of distribution of REP sequences in genomes of the examined isolates. The arithmetic averages clustering algorithm (UPGMA) statistical analysis of fingerprints with the use of the Jaccard similarity coefficient differentiated E. coli isolates into three similarity groups containing various numbers of isolates. The groups comprised isolates derived from two, three and four species of the source animals. The isolates derived from each source segregated in the dendrogram in a different way, both within the similarity groups and among them, indicating an individual repertoire of E. coli in the examined species of animals. The similarity relations among E. coli derived from the same source, illustrated in a dendrogram with a number of subclusters of a low mutual similarity (< or = 20%), indicated an essential interstrain differentiation in terms of the distribution of REP sequences. Our results confirmed the hypothesis of the oligoclonal characters of populations obtained from particular sources. The rep-PCR fingerprinting method with REP primers is simple and highly differentiating and can be recommended for use in explorations of large groups of animals and monitoring the variability of strains.

Department of Microbiology and Genetics Institute of Biotechnology and Environmental Protection University of Zielona Góra 65 561 Zielona Góra Poland

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Clin Microbiol Rev. 1994 Apr;7(2):174-84 PubMed

Vet Microbiol. 1995 Jan;43(1):13-9 PubMed

Nucleic Acids Res. 1990 Jul 11;18(13):3941-52 PubMed

Folia Microbiol (Praha). 2002;47(4):450-4 PubMed

Mol Microbiol. 1991 Apr;5(4):825-34 PubMed

Curr Opin Microbiol. 1999 Jun;2(3):306-11 PubMed

Appl Environ Microbiol. 1992 Jul;58(7):2180-7 PubMed

Nucleic Acids Res. 1990 Nov 25;18(22):6503-8 PubMed

Int J Syst Evol Microbiol. 2000 Mar;50 Pt 2:665-77 PubMed

Appl Environ Microbiol. 1994 Jul;60(7):2286-95 PubMed

Microbiol Mol Biol Rev. 2001 Mar;65(1):119-30 PubMed

Appl Environ Microbiol. 1998 Jun;64(6):2096-104 PubMed

Appl Environ Microbiol. 2000 Jun;66(6):2572-7 PubMed

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