Comparison of genetic variability between Czech and foreign isolates of phytopathogenic bacteria Clavibacter michiganensis subsp. sepedonicus by Rep-PCR technique
Language English Country United States Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
12422527
DOI
10.1007/bf02818707
Knihovny.cz E-resources
- MeSH
- Actinomycetales genetics isolation & purification pathogenicity MeSH
- DNA, Bacterial genetics isolation & purification MeSH
- Genetic Variation MeSH
- Plant Diseases microbiology MeSH
- Polymerase Chain Reaction methods MeSH
- Repetitive Sequences, Nucleic Acid MeSH
- Solanum tuberosum microbiology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- DNA, Bacterial MeSH
Repetitive-sequence-based polymerase chain reaction (Rep-PCR) method was used for analysis of genetic variability among bacterial populations from different world locations. Collection of 26 Czech and 13 foreign strains of Clavibacter michiganensis subsp. sepedonicus was amplified using BOX primer targeting to repetitive motif occurring in eubacterial genomes. Genetic fingerprints were visually compared and statistically evaluated by cluster analysis. Genetic similarity was estimated to be approximately 80% among all tested strains. Populations of these bacteria seem to be highly homogeneous; potential influence of geographic origin was not confirmed.
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