Cutting edge: TCR delta gene is frequently rearranged in adult B lymphocytes
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Validation Study
- MeSH
- Precursor Cell Lymphoblastic Leukemia-Lymphoma genetics immunology pathology MeSH
- HT29 Cells MeSH
- Adult MeSH
- Genetic Markers immunology MeSH
- Gene Rearrangement, delta-Chain T-Cell Antigen Receptor * MeSH
- HeLa Cells MeSH
- Hematopoietic Stem Cells immunology metabolism pathology MeSH
- Cloning, Molecular MeSH
- Humans MeSH
- Tumor Cells, Cultured MeSH
- B-Lymphocyte Subsets immunology metabolism MeSH
- Polymerase Chain Reaction MeSH
- Receptors, Antigen, T-Cell, gamma-delta analysis biosynthesis genetics MeSH
- Sequence Analysis, DNA MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Validation Study MeSH
- Names of Substances
- Genetic Markers MeSH
- Receptors, Antigen, T-Cell, gamma-delta MeSH
TCR gene rearrangement generates diversity of T lymphocytes by V(D)J recombination. Ig genes are rearranged in B cells using the same enzyme machinery. Physiologically, TCR gene is postulated to rearrange exclusively in T lineage, but malignant B precursor lymphoblasts contain rearranged TCR genes in most patients. Several mechanisms by which malignant cells break the regulation of V(D)J recombination have been proposed. In this study we show that incomplete TCR delta rearrangements V2-D3 and D2-D3 occur each in up to 16% alleles in B lymphocytes of all healthy donors studied, but complete VDJ rearrangement was negative at the sensitivity limit of 1%. Data are based on real-time quantitative PCR validated by PAGE and sequencing of the cloned products. Therefore, TCR genes rearrange not exclusively in T lineage. This study opens up further questions regarding the exact extent of the "cross-lineage" TCR or Ig rearrangements in normal lymphocytes, specific subsets in which the cross-lineage rearrangements occur, and the physiological importance of these rearrangements.
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