Incorporation of iron into Tritrichomonas foetus cell compartments reveals ferredoxin as a major iron-binding protein in hydrogenosomes
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
12855742
DOI
10.1099/mic.0.26122-0
Knihovny.cz E-zdroje
- MeSH
- druhová specificita MeSH
- Entamoeba histolytica genetika metabolismus MeSH
- ferredoxiny chemie genetika metabolismus MeSH
- Giardia lamblia genetika metabolismus MeSH
- kinetika MeSH
- molekulární sekvence - údaje MeSH
- molekulová hmotnost MeSH
- mutace MeSH
- organely metabolismus MeSH
- proteiny vázající železo chemie genetika metabolismus MeSH
- protozoální DNA genetika MeSH
- protozoální geny MeSH
- protozoální proteiny chemie genetika metabolismus MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- sekvenční homologie aminokyselin MeSH
- skot MeSH
- Tritrichomonas foetus genetika metabolismus patogenita MeSH
- železo metabolismus MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- ferredoxiny MeSH
- proteiny vázající železo MeSH
- protozoální DNA MeSH
- protozoální proteiny MeSH
- železo MeSH
The intracellular transport of iron and its incorporation into organelles are poorly understood processes in eukaryotes and virtually unknown in parasitic protists. The transport of iron is of particular interest in trichomonads, which possess hydrogenosomes instead of mitochondria. The metabolic functions of hydrogenosomes, which contain a specific set of FeS proteins, entirely depend on iron acquisition. In this work the incorporation of iron into the cattle parasite Tritrichomonas foetus was monitored. Iron was efficiently taken up from (59)Fe-nitrilotriacetic acid and accumulated in the cytosol (88.9 %) and hydrogenosomes (4.7 % of the total radioactivity). Using atomic absorption spectrophotometry, an unusually high steady-state iron concentration in hydrogenosomes was determined [54.4+/-1.1 nmol Fe (mg protein)(-1)]. The concentration of iron in the cytosol was 13.4+/-0.5 nmol Fe (mg protein)(-1). Qualitative analysis of incorporated iron was performed using native gradient PAGE. The majority of the (59)Fe in the cytosol appeared as the labile-iron pool, which represents weakly bound iron associated with compounds of molecular mass ranging from 5000 to 30000 Da. Ferritin was not observed in Tt. foetus, nor in two other anaerobic protists, Entamoeba histolytica and Giardia intestinalis. Analysis of Tt. foetus hydrogenosomes showed at least nine iron-binding compounds, which were absent in metronidazole-resistant mutants. The major iron-binding compound was identified as [2Fe-2S] ferredoxin of the adrenodoxin type.
Citace poskytuje Crossref.org
GENBANK
AF545472
