Effect of acute acidosis on protein and amino acid metabolism in rats
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
14512030
DOI
10.1016/s0261-5614(03)00041-4
PII: S0261561403000414
Knihovny.cz E-resources
- MeSH
- Acidosis metabolism MeSH
- Acute Disease MeSH
- Amino Acids blood metabolism MeSH
- Muscle, Skeletal metabolism MeSH
- Rats MeSH
- Leucine metabolism MeSH
- Oxidation-Reduction MeSH
- Rats, Wistar MeSH
- Proteins metabolism MeSH
- Carbon Radioisotopes MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Amino Acids MeSH
- Leucine MeSH
- Proteins MeSH
- Carbon Radioisotopes MeSH
BACKGROUND & AIMS: Metabolic acidosis is a common finding in critical illness. The aim of the present study was to evaluate acute acidosis as a signal that induces changes in protein metabolism. METHODS: In the first study, Wistar rats were infused for 6h with HCl or saline resulting in blood pH7.30+/-0.03 and 7.46+/-0.02, respectively. The whole body protein metabolism was evaluated using L-[1-(14)C]leucine. In the second study, soleus and extensor digitorum longus muscles from normal rats were incubated in medium, pH7.4, 7.3 or 7.0. Protein metabolism was evaluated using L-[1-(14)C]leucine and tyrosine release. RESULTS: In the in vivo study we observed increased protein turnover-protein synthesis, proteolysis and leucine oxidation and more negative protein balance in rats with acidosis. There was no change in protein synthesis in gastrocnemius muscle. We observed an increase in plasma levels of most amino acids including branched-chain amino acids and a decrease in intracellular amino acid pool in skeletal muscle. In vitro decrease in pH of 0.1 had no effect on protein metabolism, decrease of 0.4 decreased protein turnover and leucine oxidation. CONCLUSION: Acute metabolic acidosis is a protein wasting condition. Direct effect of acidosis on skeletal muscle is under condition in vivo modified by neurohumoral regulations.
References provided by Crossref.org
Effects of branched-chain amino acids on muscles under hyperammonemic conditions
The dose-dependent effects of endotoxin on protein metabolism in two types of rat skeletal muscle
Muscle wasting in animal models of severe illness
Protein metabolism in slow- and fast-twitch skeletal muscle during turpentine-induced inflammation
Effects of proteasome inhibitors MG132, ZL3VS and AdaAhx3L3VS on protein metabolism in septic rats
Acute effects of acidosis on protein and amino acid metabolism in perfused rat liver
