Altered blood-brain barrier permeability and its effect on the distribution of Evans blue and sodium fluorescein in the rat brain applied by intracarotid injection
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
14535837
Knihovny.cz E-resources
- MeSH
- Carotid Artery, Common MeSH
- Carotid Artery, Internal MeSH
- Brain Edema physiopathology MeSH
- Evans Blue administration & dosage pharmacokinetics MeSH
- Fluorescein administration & dosage pharmacokinetics MeSH
- Microscopy, Fluorescence MeSH
- Dentate Gyrus blood supply metabolism MeSH
- Blood-Brain Barrier drug effects physiopathology MeSH
- Hippocampus blood supply metabolism MeSH
- Hyponatremia physiopathology MeSH
- Injections, Intra-Arterial MeSH
- Injections, Intraperitoneal MeSH
- Capillary Permeability drug effects MeSH
- Rats MeSH
- Mannitol administration & dosage pharmacology MeSH
- Brain Chemistry MeSH
- Brain blood supply metabolism MeSH
- Cerebral Cortex blood supply metabolism MeSH
- Osmotic Pressure MeSH
- Rats, Wistar MeSH
- Water administration & dosage pharmacology MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Evans Blue MeSH
- Fluorescein MeSH
- Mannitol MeSH
- Water MeSH
The aim was to study the blood-brain permeability according to the distribution in the rat brain of Evans blue (EB) and sodium fluorescein (NaFl) administered by an intracarotid injection. Eighteen animals were divided into six groups according to the state of the blood-brain barrier (BBB) at the moment when the dyes were being applied. In the first two groups, the BBB was intact, in groups 3 and 4 the barrier had been opened osmotically prior to the application of the dyes, and in groups 5 and 6 a cellular edema was induced by hyperhydration before administration of the dyes. The intracellular and extracellular distribution of the dyes was studied by fluorescence microscopy. The histological picture thus represented the morphological correlate of the way BBB permeability had been changed before the application of the dyes.
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