The effects of hydrocortisone on rat heart muscarinic and adrenergic alpha 1, beta 1 and beta 2 receptors, propranolol-resistant binding sites and on some subsequent steps in intracellular signalling
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
- MeSH
- adrenergní receptory účinky léků metabolismus fyziologie MeSH
- alfa-1-adrenergní receptory účinky léků metabolismus fyziologie MeSH
- beta blokátory farmakologie MeSH
- beta-1-adrenergní receptory účinky léků metabolismus fyziologie MeSH
- beta-2-adrenergní receptory účinky léků metabolismus fyziologie MeSH
- beta-adrenergní receptory účinky léků metabolismus fyziologie MeSH
- glukokortikoidy aplikace a dávkování farmakologie MeSH
- hydrokortison aplikace a dávkování farmakologie MeSH
- injekce subkutánní MeSH
- krysa rodu Rattus MeSH
- myokard metabolismus MeSH
- potkani Wistar MeSH
- propanolaminy farmakologie MeSH
- propranolol farmakologie MeSH
- radioligandová zkouška MeSH
- receptory muskarinové účinky léků metabolismus fyziologie MeSH
- signální transdukce účinky léků MeSH
- srdeční komory účinky léků MeSH
- vazebná místa MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- adrenergic beta-4 receptor MeSH Prohlížeč
- adrenergní receptory MeSH
- alfa-1-adrenergní receptory MeSH
- beta blokátory MeSH
- beta-1-adrenergní receptory MeSH
- beta-2-adrenergní receptory MeSH
- beta-adrenergní receptory MeSH
- CGP 12177 MeSH Prohlížeč
- glukokortikoidy MeSH
- hydrokortison MeSH
- propanolaminy MeSH
- propranolol MeSH
- receptory muskarinové MeSH
Glucocorticoids affect the expression and density of neurotransmitter receptors in many tissues but data concerning the heart are contradictory and incomplete. We injected rats with hydrocortisone for 1-12 days and measured the densities of cardiac muscarinic receptors, alpha(1)-, beta(1)- and beta(2)-adrenoceptors and propranolol-resistant binding sites (formerly assumed to be the putative beta(4)-adrenoceptor). Some aspects of intracellular signalling were also evaluated: we measured adenylyl cyclase activity (basal, isoprenaline- and forskolin-stimulated and carbachol-inhibited), the coupling between muscarinic receptors and G proteins and basal and isoprenaline-stimulated heart rate. The density of cardiac muscarinic receptors increased (in both the atria and the ventricles). The density of beta(1)-adrenoceptors increased in the atria and was little changed in the ventricles. The density of beta(2)-adrenoceptors increased in both the atria and the ventricles. The number of alpha(1)-adrenoceptors decreased initially, followed by a transient increase in the atria and did not change in the ventricles. The density of propranolol-resistant binding sites first increased and then diminished in the atria and did not change in the ventricles. Although there were noticeable changes in receptor densities, the stimulatory and inhibitory effects on adenylyl cyclase, basal and isoprenaline-stimulated heart rate and the coupling between muscarinic receptors and G proteins were not significantly altered. This may indicate that changes in receptor densities might be one of the mechanisms maintaining stable functional output.
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