Determination of isosorbide-5-mononitrate in human plasma by high-resolution gas chromatography
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu klinické zkoušky, časopisecké články
PubMed
14698265
DOI
10.1016/j.jchromb.2003.10.069
PII: S1570023203009127
Knihovny.cz E-zdroje
- MeSH
- chromatografie plynová MeSH
- dimethylpolysiloxany MeSH
- fluoreny MeSH
- indikátory a reagencie MeSH
- isosorbiddinitrát analogy a deriváty krev farmakokinetika MeSH
- lidé MeSH
- referenční standardy MeSH
- reprodukovatelnost výsledků MeSH
- řízení kvality MeSH
- roztoky MeSH
- silikony MeSH
- vazodilatancia krev farmakokinetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- klinické zkoušky MeSH
- Názvy látek
- 9-fluorenone MeSH Prohlížeč
- baysilon MeSH Prohlížeč
- dimethylpolysiloxany MeSH
- fluoreny MeSH
- indikátory a reagencie MeSH
- isosorbiddinitrát MeSH
- isosorbide-5-mononitrate MeSH Prohlížeč
- roztoky MeSH
- silikony MeSH
- vazodilatancia MeSH
A method for the determination of isosorbide-5-mononitrate (5-ISMN) in human plasma by capillary gas chromatography with electron-capture detection was developed and applied to clinical samples. 9-Fluorenone was used as an internal standard, ethyl acetate was employed for liquid-liquid extraction. The advantage of the extraction procedure is the possibility of a direct injection of the plasma extract, without solvent removal/reconstitution of the sample. The precision and accuracy of the method were satisfactory in the concentration range 10-1600 ng/ml. The lower limit of quantification was 10 ng/ml.
Citace poskytuje Crossref.org
