Characterization of the cryptic plasmid pCC1 from Corynebacterium callunae and its use for vector construction
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
14711530
DOI
10.1016/j.plasmid.2003.09.002
PII: S0147619X03000799
Knihovny.cz E-resources
- MeSH
- Amino Acid Motifs MeSH
- Corynebacterium genetics MeSH
- Genetic Vectors * MeSH
- Nucleic Acid Conformation MeSH
- Conserved Sequence MeSH
- Plasmids * MeSH
- Sequence Analysis, DNA MeSH
- Sequence Analysis, Protein MeSH
- Sequence Alignment MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The complete nucleotide sequence of the cryptic plasmid pCC1 from Corynebacterium callunae (4109 bp) was determined. DNA sequence analysis revealed five open reading frames longer than 200 bp. One of the deduced polypeptides showed homology with the Rep proteins encoded by plasmids of the pIJ101/pJV1 family of plasmids replicating by the rolling-circle (RC) mechanism. Within this plasmid family, the Rep protein of pCC1 showed the highest degree of similarity to the Rep proteins of corynebacterial plasmids pAG3 and pBL1. These data suggest that the plasmid pCC1 replicates by the RC mechanism. The Escherichia coli/Corynebacterium glutamicum shuttle cloning vector pSCCD1, carrying the pCC1 rep gene on the 2.1-kb DNA fragment and the streptomycin/spectinomycin resistance determinant, was constructed. This vector is stably maintained in population of C. glutamicum cells grown in the absence of selection pressure and it is compatible with plasmid vectors based on corynebacterial plasmids pBL1 and pSR1.
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