This review discusses methods for the single-chain antibody fragment ($cFv) generation and scFv expression systems, and describes potential applications of scFv in the therapy of viral diseases and cancer, with emphasis on intracellularly expressed scFvs (intrabodies), application of scFvs in detection and diagnostics, and their use in proteomics.

Institute of Microbiology and Immunology Faculty of Veterinary Medicine Veterinary and Pharmaceutical University 612 42 Brno Czechia

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Gene. 1995 Dec 12;166(2):273-6 PubMed

J Virol. 1996 Dec;70(12):8821-32 PubMed

J Immunol Methods. 1997 Jan 15;200(1-2):69-77 PubMed

Eur J Immunol. 2001 Jan;31(1):250-8 PubMed

Biotechnology (N Y). 1994 Apr;12(4):396-9 PubMed

J Biol Chem. 2002 Jan 4;277(1):593-601 PubMed

Antiviral Res. 2002 Oct;56(1):51-9 PubMed

J Virol Methods. 1997 Sep;67(2):221-33 PubMed

Curr Top Microbiol Immunol. 1998;234:83-96 PubMed

Immunotechnology. 1996 Sep;2(3):219-28 PubMed

Biosens Bioelectron. 2001 Dec;16(9-12):639-46 PubMed

Proc Natl Acad Sci U S A. 1995 Aug 29;92 (18):8358-62 PubMed

Proc Natl Acad Sci U S A. 1994 May 24;91(11):5075-9 PubMed

J Biol Chem. 1994 Oct 21;269(42):26267-73 PubMed

J Virol. 1996 Jun;70(6):3392-400 PubMed

Protein Expr Purif. 1999 Jun;16(1):63-9 PubMed

Gene Ther. 1997 Jan;4(1):11-5 PubMed

J Virol Methods. 2002 Dec;106(2):225-33 PubMed

Gene Ther. 1999 Apr;6(4):660-6 PubMed

Cancer Detect Prev. 1999;23(6):506-10 PubMed

Adv Drug Deliv Rev. 1998 Apr 6;31(1-2):153-170 PubMed

Biochem Biophys Res Commun. 1999 Sep 24;263(2):410-8 PubMed

Cancer Gene Ther. 2001 Aug;8(8):555-65 PubMed

Folia Microbiol (Praha). 2003;48(3):435-40 PubMed

J Biol Chem. 2003 Jan 17;278(3):1433-42 PubMed

Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7978-82 PubMed

J Biol Chem. 2002 Aug 30;277(35):32036-45 PubMed

Gene Ther. 2001 Mar;8(5):408-18 PubMed

Nat Genet. 1999 Jan;21(1 Suppl):33-7 PubMed

Methods Enzymol. 1989;178:497-515 PubMed

Nat Biotechnol. 2000 Sep;18(9):989-94 PubMed

Expert Opin Biol Ther. 2001 Sep;1(5):845-55 PubMed

Clin Cancer Res. 2000 Aug;6(8):3081-7 PubMed

Cell. 1987 Mar 13;48(5):899-907 PubMed

J Mol Biol. 2002 Jun 28;320(1):1-10 PubMed

Arch Virol. 2002 Oct;147(10):2025-37 PubMed

Gene. 1988 Dec 20;73(2):305-18 PubMed

Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):5932-6 PubMed

Mol Med. 1997 Feb;3(2):96-110 PubMed

FEMS Immunol Med Microbiol. 2002 Nov 15;34(3):237-44 PubMed

Phytopathology. 2000 Feb;90(2):183-90 PubMed

Anal Biochem. 2002 Oct 1;309(1):27-34 PubMed

Anal Biochem. 1997 Jul 1;249(2):219-27 PubMed

Curr Opin Immunol. 1993 Apr;5(2):256-62 PubMed

Nature. 1984 Sep 6-11;311(5981):33-8 PubMed

Anticancer Res. 2000 Nov-Dec;20(6A):4067-71 PubMed

Proc Natl Acad Sci U S A. 1998 May 26;95(11):6157-62 PubMed

Eur J Biochem. 2000 Feb;267(4):1196-205 PubMed

Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):805-10 PubMed

Nature. 1988 Sep 29;335(6189):395-6 PubMed

Clin Diagn Lab Immunol. 1998 Sep;5(5):636-44 PubMed

Mol Ther. 2002 Jan;5(1):33-41 PubMed

Adv Drug Deliv Rev. 1998 Apr 6;31(1-2):89-104 PubMed

J Immunol Methods. 2001 Nov 1;257(1-2):175-84 PubMed

Curr Opin Biotechnol. 1998 Feb;9(1):102-8 PubMed

Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7889-93 PubMed

Nucleic Acids Res. 2000 Aug 1;28(15):E72 PubMed

J Mol Biol. 2000 Feb 11;296(1):57-86 PubMed

J Biotechnol. 2000 Jan 21;76(2-3):157-63 PubMed

Biotechnology (N Y). 1991 Feb;9(2):165-9 PubMed

J Hepatol. 2002 Nov;37(5):660-8 PubMed

J Immunol Methods. 1999 Dec 10;231(1-2):223-38 PubMed

J Mol Biol. 2002 Feb 1;315(5):1063-73 PubMed

Science. 1988 Oct 21;242(4877):423-6 PubMed

Nature. 1993 Dec 2;366(6454):469-72 PubMed

J Mol Biol. 1987 Aug 20;196(4):947-50 PubMed

Science. 1988 May 20;240(4855):1038-41 PubMed

Hum Gene Ther. 2000 Feb 10;11(3):389-401 PubMed

J Clin Oncol. 1998 Oct;16(10 ):3270-8 PubMed

J Virol. 2000 Jun;74(12):5712-5 PubMed

J Immunol Methods. 1999 Nov 19;230(1-2):121-30 PubMed

J Immunol Methods. 1998 Jul 1;216(1-2):165-81 PubMed

J Immunol Methods. 2002 May 1;263(1-2):149-67 PubMed

Nature. 1991 Aug 15;352(6336):624-8 PubMed

Nature. 1990 Dec 6;348(6301):552-4 PubMed

J Immunol Methods. 1995 Oct 26;186(2):245-55 PubMed

J Hum Virol. 2000 Jan-Feb;3(1):6-15 PubMed

Nature. 1975 Aug 7;256(5517):495-7 PubMed

Annu Rev Microbiol. 1997;51:257-83 PubMed

FEBS Lett. 1997 Sep 15;414(3):521-6 PubMed

Plant Mol Biol. 1994 Nov;26(4):1023-30 PubMed

J Mol Biol. 1993 Apr 5;230(3):812-23 PubMed

Proc Natl Acad Sci U S A. 1997 May 13;94(10):4937-42 PubMed

J Virol. 1997 Sep;71(9):6486-94 PubMed

J Virol. 1996 May;70(5):3290-7 PubMed

J Virol Methods. 1999 Aug;81(1-2):159-68 PubMed

Biotechnology (N Y). 1992 Jul;10(7):790-4 PubMed