Fluorescent labelled thiourea-bridged glycodendrons
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Killer Cells, Natural cytology metabolism MeSH
- Fluorescence MeSH
- Galactose chemistry metabolism MeSH
- Glycoconjugates chemical synthesis chemistry MeSH
- Microscopy, Confocal MeSH
- Magnetic Resonance Spectroscopy MeSH
- Molecular Structure MeSH
- Mice MeSH
- Spleen cytology metabolism MeSH
- Thiourea chemistry MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Galactose MeSH
- Glycoconjugates MeSH
- Thiourea MeSH
GlcNAc-coated glycodendrimers, which are polyvalent glycomimetics, display strong in vitro affinity for the rat natural killer cell protein-1A (NKR-P1A), a C-type lectin-like receptor of natural killer (NK) cells in rats, humans and some strains of mice. Administration of these compounds in vivo results in a substantial increase in the antitumour activity with involvement of the natural cell immunity. To clarify the in vitro and in vivo fate of these molecules, we synthesized labelled glycodendron analogues of the previously studied glycodendrimers. Labelling with fluorescent tags enabled the localization of the glycodendrons in white blood cells, tumours and other tissues by using different imaging techniques such as fluorescence and confocal microscopy. These studies are useful for probing the mechanism of action and fate of artificial ligands and the cell receptors involved.
References provided by Crossref.org
Nkrp1 family, from lectins to protein interacting molecules
