The binding of monosaccharides and short peptides to lymphocyte receptors (human CD69 and rat NKR-P1A) was first reported in 1994 and then in a number of subsequent publications. Based on this observation, numerous potentially high-affinity saccharide ligands have been synthesized over the last two decades in order to utilize their potential in antitumor therapy. Due to significant inconsistencies in their reported binding properties, we decided to re-examine the interaction between multiple ligands and CD69 or NKR-P1A. Using NMR titration and isothermal titration calorimetry we were unable to detect the binding of the tested ligands such as N-acetyl-D-hexosamines and oligopeptides to both receptors, which contradicts the previous observations published in more than twenty papers over the last fifteen years.

Institute of Microbiology Academy of Sciences of the Czech Republic Vídeňská 1083 Prague 4 CZ14220 Czech Republic

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Report of the Joint Ethical Committee of the Institute of Microbiology, Prague and Charles University in Prague. English Translation Can Be Found in the Supplementary Material. [(accessed on 15 January 2014)]. Available online: http://www.biomed.cas.cz/mbu/doc/VyjadreniEK.PDF.

Structure of the human NK cell NKR-P1:LLT1 receptor:ligand complex reveals clustering in the immune synapse

Nkrp1 family, from lectins to protein interacting molecules