Occurrence of plasmid-mediated ampicillin resistance among enterobacteria from the ovine rumen
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
15227795
DOI
10.1007/bf02931399
Knihovny.cz E-zdroje
- MeSH
- ampicilin farmakologie MeSH
- antibakteriální látky farmakologie MeSH
- bachor mikrobiologie MeSH
- beta-laktamasy genetika MeSH
- Enterobacteriaceae účinky léků izolace a purifikace MeSH
- Escherichia coli genetika MeSH
- feces mikrobiologie MeSH
- konjugace genetická MeSH
- ovce mikrobiologie MeSH
- přenos genů horizontální MeSH
- R-plasmidy * MeSH
- rezistence na ampicilin genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- ampicilin MeSH
- antibakteriální látky MeSH
- beta-laktamasy MeSH
Seasonal samplings of rumen and fecal populations of Enterobacteriacae from sheep digestive tract were done to elucidate potential occurrence and spreading of antibiotic resistance in the environment. Thus 350 rumen and fecal isolates were tested for ampicillin (Amp) resistance in single sampling. Low frequency of Amp resistance (from 0 to 15%) was observed. The occurrence of tem1 encoded Amp resistance confirmed by PCR was observed among both rumen and fecal isolates. The small tem1 carrying plasmid and its transfer (mobilization) was detected and partially characterized after conjugation to laboratory Escherichia coli strain.
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