P-glycoprotein expression and distribution in the rat placenta during pregnancy
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15279876
DOI
10.1016/j.reprotox.2004.04.014
PII: S0890623804000978
Knihovny.cz E-resources
- MeSH
- ATP-Binding Cassette Sub-Family B Member 4 MeSH
- ATP-Binding Cassette Transporters biosynthesis genetics MeSH
- Chorion metabolism MeSH
- Immunohistochemistry MeSH
- Rats MeSH
- Membranes drug effects metabolism MeSH
- RNA, Messenger biosynthesis MeSH
- Antibodies, Monoclonal MeSH
- ATP Binding Cassette Transporter, Subfamily B, Member 1 biosynthesis MeSH
- ATP Binding Cassette Transporter, Subfamily B biosynthesis genetics MeSH
- Placenta metabolism MeSH
- Placentation MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Rats, Wistar MeSH
- Pregnancy, Animal metabolism MeSH
- Pregnancy MeSH
- Blotting, Western MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Pregnancy MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- ATP-Binding Cassette Transporters MeSH
- RNA, Messenger MeSH
- Antibodies, Monoclonal MeSH
- multidrug resistance protein 3 MeSH Browser
- ATP Binding Cassette Transporter, Subfamily B, Member 1 MeSH
- ATP Binding Cassette Transporter, Subfamily B MeSH
P-glycoprotein (P-gp) is a drug efflux transporter that limits the entry of various potentially toxic drugs and xenobiotics into the fetus and is thus considered a placental protective mechanism. In this study, P-gp expression was investigated in the rat chorioallantoic placenta over the course of pregnancy. Three methods have been employed: real-time RT-PCR, western blotting and immunohistochemistry. The expression of mdr1a and mdr1b genes was demonstrated as early as on the 11th gestation day (gd) and increased with advancing gestation. Western blotting analysis revealed the presence of P-gp in the rat placenta starting from gd 13 onwards. P-gp was localized in the developing labyrinth zone of the placenta on gd 13; from gd 15 up to the term P-gp was seen as a dot like continuous line in the syncytiotrophoblast layers. Our data confirm the presence of P-gp in the rat chorioallantoic placenta starting soon after its development, which may signify the involvement of P-gp in transplacental pharmacokinetics during the whole period of placental maturing.
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