The analysis of correlations between drug resistance and clinical/laboratory measures found in a group of children with all treated by ALL-BFM 90 protocol
Language English Country France Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15562558
Knihovny.cz E-resources
- MeSH
- Precursor Cell Lymphoblastic Leukemia-Lymphoma drug therapy MeSH
- Asparaginase therapeutic use MeSH
- Coloring Agents MeSH
- Drug Resistance, Neoplasm * MeSH
- Cyclophosphamide therapeutic use MeSH
- Cytarabine therapeutic use MeSH
- Daunorubicin therapeutic use MeSH
- Child MeSH
- Remission Induction MeSH
- Humans MeSH
- Mercaptopurine therapeutic use MeSH
- Methotrexate therapeutic use MeSH
- Adolescent MeSH
- Multivariate Analysis MeSH
- Predictive Value of Tests MeSH
- Prednisone therapeutic use MeSH
- Child, Preschool MeSH
- Antineoplastic Combined Chemotherapy Protocols therapeutic use MeSH
- Sex Factors MeSH
- In Vitro Techniques MeSH
- Tetrazolium Salts MeSH
- Thiazoles MeSH
- Age Factors MeSH
- Vincristine therapeutic use MeSH
- Check Tag
- Child MeSH
- Humans MeSH
- Adolescent MeSH
- Male MeSH
- Child, Preschool MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Asparaginase MeSH
- Coloring Agents MeSH
- Cyclophosphamide MeSH
- Cytarabine MeSH
- Daunorubicin MeSH
- Mercaptopurine MeSH
- Methotrexate MeSH
- Prednisone MeSH
- Tetrazolium Salts MeSH
- Thiazoles MeSH
- thiazolyl blue MeSH Browser
- Vincristine MeSH
This study was designed to assess the predictive value of an MTT (3-[4,5-dimethylthiazol-2,5-diphenyl] tetrazolium bromide) in vitro assay for the evaluation of leukemic cell resistance/sensitivity to cytotoxic drugs, and to compare these results with clinical and laboratory parameters in cases of childhood acute lymphoblastic leukemia (ALL).The chemoresistance of leukemic cells was ascertained by means of an MTT assay in 32 previously untreated children with ALL. The children were treated using the protocol ALL-BFM 90. Statistical correlations were made between in vitro drug resistance to anti-cancer drugs: prednisolone (PRED), vincristine (VCR),daunorubicin (DNR), etoposide (VP-16) and cytosine arabinoside (ARA-C) and in vivo clinical and laboratory parameters: age, sex, risk factor (RF), leukocytes (WBC)and absolute number of blast cells (BC) at diagnosis (BC0), BC at day 8 (BC8), the percentage of blast cells in bone marrow at day 15 (BM15) and at day 33 (BM33),and leukocyte surface antigens CD3, CD4, CD5, CD8, CD10, CD19, CD20, HLADR.
