Magnetic techniques for the isolation and purification of proteins and peptides
Status PubMed-not-MEDLINE Jazyk angličtina Země Velká Británie, Anglie Médium electronic
Typ dokumentu časopisecké články
PubMed
15566570
PubMed Central
PMC544596
DOI
10.1186/1477-044x-2-7
PII: 1477-044X-2-7
Knihovny.cz E-zdroje
- Publikační typ
- časopisecké články MeSH
Isolation and separation of specific molecules is used in almost all areas of biosciences and biotechnology. Diverse procedures can be used to achieve this goal. Recently, increased attention has been paid to the development and application of magnetic separation techniques, which employ small magnetic particles. The purpose of this review paper is to summarize various methodologies, strategies and materials which can be used for the isolation and purification of target proteins and peptides with the help of magnetic field. An extensive list of realised purification procedures documents the efficiency of magnetic separation techniques.
Zobrazit více v PubMed
Schuster M, Wasserbauer E, Ortner C, Graumann K, Jungbauer A, Hammerschmid F, Werner G. Short cut of protein purification by integration of cell-disrupture and affinity extraction. Bioseparation. 2000;9:59–67. doi: 10.1023/A:1008135913202. PubMed DOI
Hofmann I, Schnolzer M, Kaufmann I, Franke WW. Symplekin, a constitutive protein of karyo- and cytoplasmic particles involved in mRNA biogenesis in Xenopus laevis oocytes. Mol Biol Cell. 2002;13:1665–1676. doi: 10.1091/mbc.01-12-0567. PubMed DOI PMC
Alche JD, Dickinson K. Affinity chromatographic purification of antibodies to a biotinylated fusion protein expressed in Escherichia coli. Protein Expr Purif. 1998;12:138–143. doi: 10.1006/prep.1997.0824. PubMed DOI
Teotia S, Gupta MN. Purification of alpha-amylases using magnetic alginate beads. Appl Biochem Biotechnol. 2001;90:211–220. doi: 10.1385/ABAB:90:3:211. PubMed DOI
Safarik I, Safarikova M. Use of magnetic techniques for the isolation of cells. J Chromatogr B Biomed Sci Appl. 1999;722:33–53. doi: 10.1016/S0378-4347(98)00338-7. PubMed DOI
Sinclair B. To bead or not to bead: Applications of magnetic bead technology. Scientist. 1998;12:17.
Bruce IJ, Taylor J, Todd M, Davies MJ, Borioni E, Sangregorio C, Sen T. Synthesis, characterisation and application of silica-magnetite nanocomposites. J Magn Magn Mater. 2004;284:145–160. doi: 10.1016/j.jmmm.2004.06.032. DOI
Weetall HH, Lee MJ. Antibodies immobilized on inorganic supports. Appl Biochem Biotechnol. 1989;22:311–330. PubMed
Safarik I, Safarikova M. Batch isolation of hen egg white lysozyme with magnetic chitin. J Biochem Biophys Methods. 1993;27:327–330. doi: 10.1016/0165-022X(93)90013-E. PubMed DOI
Safarikova M, Roy I, Gupta MN, Safarik I. Magnetic alginate microparticles for purification of α-amylases. J Biotechnol. 2003;105:255–260. doi: 10.1016/j.jbiotec.2003.07.002. PubMed DOI
Tanyolac D, Ozdural AR. A new low cost magnetic material: magnetic polyvinylbutyral microbeads. React Funct Polym. 2000;43:279–286. doi: 10.1016/S1381-5148(99)00054-1. DOI
Nixon L, Koval CA, Noble RD, Slaff GS. Preparation and characterization of novel magnetite-coated ion-exchange particles. Chem Mater. 1992;4:117–121.
Mosbach K, Andersson L. Magnetic ferrofluids for preparation of magnetic polymers and their application in affinity chromatography. Nature. 1977;270:259–261. PubMed
Hirschbein BL, Whitesides GM. Affinity separation of enzymes from mixtures containing suspended solids. Comparisons of magnetic and nonmagnetic techniques. Appl Biochem Biotechnol. 1982;7:157–176. PubMed
Rocha FM, de Pinho SC, Zollner RL, Santana MHA. Preparation and characterization of affinity magnetoliposomes useful for the detection of antiphospholipid antibodies. J Magn Magn Mater. 2001;225:101–108. doi: 10.1016/S0304-8853(00)01236-1. DOI
Zollner TCA, Zollner RD, de Cuyper M, Santana MHA. Adsorption of isotype "E" antibodies on affinity magnetoliposomes. J Dispersion Sci Technol. 2003;24:615–622. doi: 10.1081/DIS-120021818. DOI
Bucak S, Jones DA, Laibinis PE, Hatton TA. Protein separations using colloidal magnetic nanoparticles. Biotechnol Progr. 2003;19:477–484. doi: 10.1021/bp0200853. PubMed DOI
Lee KB, Park S, Mirkin CA. Multicomponent magnetic nanorods for biomolecular separations. Angew Chem – Int Edit. 2004;43:3048–3050. doi: 10.1002/anie.200454088. PubMed DOI
Safarik I, Ptackova L, Safarikova M. Large-scale separation of magnetic bioaffinity adsorbents. Biotechnol Lett. 2001;23:1953–1956. doi: 10.1023/A:1013742002533. DOI
Schafer F, Romer U, Emmerlich M, Blumer J, Lubenow H, Steinert K. Automated high-throughput purification of 6xHis-tagged proteins. J Biomol Tech. 2002;13:131–142. PubMed PMC
Lochmuller CH, Ronsick CS, Wigman LS. Fluidized-bed separators reviewed: a low pressure drop approach to column chromatography. Prep Chromatogr. 1988;1:93–108.
Burns MA, Graves DJ. Continuous affinity chromatography using a magnetically stabilized fluidized bed. Biotechnol Progr. 1985;1:95–103. PubMed
Chetty AS, Burns MA. Continuous protein separations in a magnetically stabilized fluidized bed using nonmagnetic supports. Biotechnol Bioeng. 1991;38:963–971. doi: 10.1002/bit.260380902. PubMed DOI
Wikstrom P, Flygare S, Grondalen A, Larsson PO. Magnetic aqueous two-phase separation: a new technique to increase rate of phase-separation, using dextran-ferrofluid or larger iron oxide particles. Anal Biochem. 1987;167:331–339. doi: 10.1016/0003-2697(87)90173-4. PubMed DOI
Larsson P-O. Magnetically enhanced phase separation. Meth Enzymol. 1994;228:112–117. PubMed
Safarik I, Safarikova M. Biologically active compounds and xenobiotics: Magnetic affinity separations. In: Wilson ID, Adlard RR, Poole CF, Cook MR, editor. Encyclopedia of Separation Science. London: Academic Press; 2000. pp. 2163–2170.
Safarik I, Safarikova M. Overview of magnetic separations used in biochemical and biotechnological applications. In: Hafeli U, Schutt W, Teller J, Zborowski M, editor. Scientific and Clinical Applications of Magnetic Carriers. New York and London: Plenum Press; 1997. pp. 323–340.
Safarikova M, Safarik I. The application of magnetic techniques in biosciences. Magn Electr Sep. 2001;10:223–252.
Saiyed ZM, Telang SD, Ramchand CN. Application of magnetic techniques in the field of drug discovery and biomedicine. BioMagn Res Technol. 2003;1:2. doi: 10.1186/1477-044X-1-2. PubMed DOI PMC
Yaneva M, Tempst P. Affinity capture of specific DNA-binding proteins for mass spectrometric identification. Anal Chem. 2003;75:6437–6448. doi: 10.1021/ac034698l. PubMed DOI
Teng CH, Ho KC, Lin YS, Chen YC. Gold nanoparticles as selective and concentrating probes for samples in MALDI MS analysis. Anal Chem. 2004;76:4337–4342. doi: 10.1021/ac049963x. PubMed DOI
Heegaard NHH, Nilsson S, Guzman NA. Affinity capillary electrophoresis: important application areas and some recent developments. J Chromatogr B. 1998;715:29–54. PubMed
Nilsson J, Stahl S, Lundeberg J, Uhlen M, Nygren PA. Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. Protein Expr Purif. 1997;11:1–16. doi: 10.1006/prep.1997.0767. PubMed DOI
Kobs G. Finding the right protein purification system. Cell Notes. 2004;9:2–5.
Gaberc-Porekar V, Menart V. Perspectives of immobilized-metal affinity chromatography. J Biochem Biophys Methods. 2001;49:335–360. doi: 10.1016/S0165-022X(01)00207-X. PubMed DOI
Frenzel A, Bergemann C, Kohl G, Reinard T. Novel purification system for 6xHis-tagged proteins by magnetic affinity separation. J Chromatogr B. 2003;793:325–329. doi: 10.1016/S1570-0232(03)00332-5. PubMed DOI
Widjojoatmodjo MN, Fluit AC, Torensma R, Verhoef J. Comparison of immunomagnetic beads coated with protein A, protein G, or goat anti-mouse immunoglobulins. J Immunol Methods. 1993;165:11–19. doi: 10.1016/0022-1759(93)90101-C. PubMed DOI
Biomagnetic Techniques in Molecular Biology. Information Booklet, Dynal, Oslo, Norway. 1998.
Fischer MB, Roeckl C, Parizek P, Schwarz HP, Aguzzi A. Binding of disease-associated prion protein to plasminogen. Nature. 2000;408:479–483. doi: 10.1038/35044100. PubMed DOI
Maissen M, Roeckl F, Glatzel M, Goldmann W, Aguzzi A. Plasminogen binds to disease-associated prion protein of multiple species. Lancet. 2001;357:2026–2028. doi: 10.1016/S0140-6736(00)05110-2. PubMed DOI
Heeboll-Nielsen A, Choe WS, Middelberg APJ, Thomas ORT. Efficient inclusion body processing using chemical extraction and high gradient magnetic fishing. Biotechnol Progr. 2003;19:887–898. doi: 10.1021/bp025553n. PubMed DOI
Safarikova M, Safarik I. Magnetic solid-phase extraction. J Magn Magn Mater. 1999;194:108–112. doi: 10.1016/S0304-8853(98)00566-6. DOI
Safarikova M, Safarik I. Magnetic solid-phase extraction of target analytes from large volumes of urine. Eur Cells Mater. 2002;3:192–195.
Goldberg M, Jenkins H, Allen T, Whitfield WG, Hutchison CJ. Xenopus lamin B3 has a direct role in the assembly of a replication competent nucleus: evidence from cell-free egg extracts. J Cell Sci. 1995;108:3451–3461. PubMed
Bell P, Scheer U. Prenucleolar bodies contain coilin and are assembled in Xenopus egg extract depleted of specific nucleolar proteins and U3 RNA. J Cell Sci. 1997;110:43–54. PubMed
Safarik I, Safarikova M. Isolation and removal of proteolytic enzymes with magnetic cross-linked erythrocytes. J Magn Magn Mater. 2001;225:169–174. doi: 10.1016/S0304-8853(00)01247-6. DOI
Adessi C, Chapel A, Vincon M, Rabilloud T, Klein G, Satre M, Garin J. Identification of major proteins associated with Dictyostelium discoideum endocytic vesicles. J Cell Sci. 1995;108:3331–3337. PubMed
Luers GH, Hartig R, Mohr H, Hausmann M, Fahimi HD, Cremer C, Volkl A. Immuno-isolation of highly purified peroxisomes using magnetic beads and continuous immunomagnetic sorting. Electrophoresis. 1998;19:1205–1210. PubMed
Brzeska M, Panhorst M, Kamp PB, Schotter J, Reiss G, Puhler A, Becker A, Bruckl H. Detection and manipulation of biomolecules by magnetic carriers. J Biotechnol. 2004;112:25–33. doi: 10.1016/j.jbiotec.2004.04.018. PubMed DOI
Hubbuch JJ, Thomas ORT. High-gradient magnetic affinity separation of trypsin from porcine pancreatin. Biotechnol Bioeng. 2002;79:301–313. doi: 10.1002/bit.10285. PubMed DOI
Hirota N, Kurashige M, Iwasaka M, Ikehata M, Uetake H, Takayama T, Nakamura H, Ikezoe Y, Ueno S, Kitazawa K. Magneto-Archimedes separation and its application to the separation of biological materials. Physica B. 2004;346–347:267–271.
Tong XD, Xue B, Sun Y. A novel magnetic affinity support for protein adsorption and purification. Biotechnol Progr. 2001;17:134–139. doi: 10.1021/bp000134g. PubMed DOI
Flygare S, Wikstrom P, Johansson G, Larsson PO. Magnetic aqueous two-phase separation in preparative applications. Enzyme Microb Technol. 1990;12:95–103. doi: 10.1016/0141-0229(90)90080-A. PubMed DOI
Murphy AS, Hoogner KR, Peer WA, Taiz L. Identification, purification, and molecular cloning of N-1-naphthylphthalmic acid-binding plasma membrane-associated aminopeptidases from Arabidopsis. Plant Physiol. 2002;128:935–950. doi: 10.1104/pp.010519. PubMed DOI PMC
Teotia S, Gupta MN. Magnetite-alginate beads for purification of some starch degrading enzymes. Mol Biotechnol. 2002;20:231–237. doi: 10.1385/MB:20:3:231. PubMed DOI
Abudiab T, Beitle RR. Preparation of magnetic immobilized metal affinity separation media and its use in the isolation of proteins. J Chromatogr A. 1998;795:211–217. doi: 10.1016/S0021-9673(97)00959-X. PubMed DOI
Barnes K, Murphy LJ, Turner AJ. Immunoseparation of membrane peptidases from pig lung membranes using magnetic beads. Biochem Soc Trans. 1994;22:S451–S451. PubMed
Dunnill P, Lilly MD. Purification of enzymes using magnetic bio-affinity materials. Biotechnol Bioeng. 1974;16:987–990. doi: 10.1002/bit.260160710. PubMed DOI
Chen D-H, Huang S-H. Fast separation of bromelain by polyacrylic acid-bound iron oxide magnetic nanoparticles. Process Biochem. 2004;39:2207–2211. doi: 10.1016/j.procbio.2003.11.014. DOI
Himeji D, Horiuchi T, Tsukamoto H, Hayashi K, Watanabe T, Harada M. Characterization of caspase-8L: a novel isoform of caspase-8 that behaves as an inhibitor of the caspase cascade. Blood. 2002;99:4070–4078. doi: 10.1182/blood.V99.11.4070. PubMed DOI
Akgol S, Denizli A. Novel metal-chelate affinity sorbents for reversible use in catalase adsorption. J Mol Catal B – Enzym. 2004;28:7–14. doi: 10.1016/j.molcatb.2003.12.010. DOI
Ghosh M, Tyagi R, Gupta MN. Preparation of trypsin free chymotrypsin. Biotechnol Tech. 1995;9:149–152. doi: 10.1007/BF00224416. DOI
O'Brien SM, Thomas ORT, Dunnill P. Non-porous magnetic chelator supports for protein recovery by immobilised metal affinity adsorption. J Biotechnol. 1996;50:13–25. doi: 10.1016/0168-1656(96)01544-1. DOI
Ljungquist C, Lundeberg J, Rasmussen AM, Hornes E, Uhlen M. Immobilization and recovery of fusion proteins and B-lymphocyte cells using magnetic separation. DNA Cell Biol. 1993;12:191–197. PubMed
Griffin T, Mosbach K, Mosbach R. Magnetic biospecific affinity adsorbents for immunoglobulin and enzyme isolation. Appl Biochem Biotechnol. 1981;6:283–292. PubMed
Ennis MP, Wisdom GB. A magnetizable solid phase for enzyme extraction. Appl Biochem Biotechnol. 1991;30:155–164. PubMed
Justesen SFL, Nielsen AH, Thomas ORT. High gradient magnetic fishing for the isolation of high-value proteins from sweet whey. Danish Biotechnology Conference VII: Vejle, Denmark. 2001.
Heeboll-Nielsen A, Justesen S, Thomas ORT. Product recovery for crude bioprocess liquors by high gradient magnetic fishing. 10th European Congress on Biotechnology: Madrid, Spain. 2001.
Betz N. Efficient purification of His-tagged proteins from insect and mammalian cells. Promega Notes. 2004;87:29–32.
Betz N. Purifying His-tagged proteins from insect and mammalian cells. Cell Notes. 2004;9:6–9.
Safarik I. Magnetic biospecific affinity adsorbents for lysozyme isolation. Biotechnol Tech. 1991;5:111–114. doi: 10.1007/BF00159981. DOI
Odabasi M, Denizli A. Cibacron blue F3GA incorporated magnetic poly(2-hydroxyethyl methacrylate) beads for lysozyme adsorption. J Appl Polym Sci. 2004;93:719–725. doi: 10.1002/app.20485. DOI
Goto M, Imamura T, Hirose T. Axial dispersion in liquid magnetically stabilized fluidized beds. J Chromatogr A. 1995;690:1–8. doi: 10.1016/0021-9673(94)00993-J. DOI
Kopacek P, Vogt R, Jindrak L, Weise C, Safarik I. Purification and characterization of the lysozyme from the gut of the soft tick Ornithodoros moubata. Insect Biochem Mol Biol. 1999;29:989–997. doi: 10.1016/S0965-1748(99)00075-2. PubMed DOI
Liao MH, Chen DH. Fast and efficient adsorption/desorption of protein by a novel magnetic nano-adsorbent. Biotechnol Lett. 2002;24:1913–1917. doi: 10.1023/A:1020956309976. DOI
Xue B, Sun Y. Protein adsorption equilibria and kinetics to a poly(vinyl alcohol)-based magnetic affinity support. J Chromatogr A. 2001;921:109–119. doi: 10.1016/S0021-9673(01)00860-3. PubMed DOI
Tong XD, Sun Y. Application of magnetic agarose support in liquid magnetically stabilized fluidized bed for protein adsorption. Biotechnol Progr. 2003;19:1721–1727. doi: 10.1021/bp030028p. PubMed DOI
Yu YH, Xue B, Sun Y, He BL. The preparation of chitosan affinity magnetic nanoparticles and their adsorption properties for proteins. Acta Polym Sinica. 2000. pp. 340–344.
Safarik I, Safarikova M, Weyda F, Mosiniewicz-Szablewska W, Slawska-Waniewska A. Ferrofluid-modified plant-based materials as adsorbents for batch separation of selected biologically active compounds and xenobiotics. J Magn Magn Mater.
Peng ZG, Hidajat K, Uddin MS. Adsorption and desorption of lysozyme on nano-sized magnetic particles and its conformational changes. Colloid Surf B – Biointerfaces. 2004;35:169–174. doi: 10.1016/j.colsurfb.2004.03.010. PubMed DOI
O'Brien SM, Sloane RP, Thomas ORT, Dunnill P. Characterisation of non-porous magnetic chelator supports and their use to recover polyhistidine-tailed T4 lysozyme from a crude E. coli extract. J Biotechnol. 1997;54:53–67. doi: 10.1016/S0168-1656(97)01688-X. PubMed DOI
Tyagi R, Gupta MN. Purification and immobilization of Aspergillus niger pectinase on magnetic latex beads. Biocatal Biotransform. 1995;12:293–298. PubMed
Hendrix PG, Hoylaerts MF, Nouwen EJ, Van de Voorde A, De Broe ME. Magnetic beads in suspension enable a rapid and sensitive immunodetection of human placental alkaline phosphatase. Eur J Clin Chem Clin Biochem. 1992;30:343–347. PubMed
Hubbuch JJ, Matthiesen DB, Hobley TJ, Thomas ORT. High gradient magnetic separation versus expanded bed adsorption: a first principle comparison. Bioseparation. 2001;10:99–112. doi: 10.1023/A:1012034923621. PubMed DOI
Engel L, Kar S, Johnson T. Rapid detection and quantitation of His-tagged proteins purified by MagneHis™ Ni-particles. Promega Notes. 2003;84:27–30.
Khng HP, Cunliffe D, Davies S, Turner NA, Vulfson EN. The synthesis of sub-micron magnetic particles and their use for preparative purification of proteins. Biotechnol Bioeng. 1998;60:419–424. PubMed
Halling PJ, Dunnill P. Recovery of free enzymes from product liquors by bio-affinity adsorption: Trypsin binding by immobilised soybean inhibitor. European J Appl Microbiol. 1979;6:195–205. doi: 10.1007/BF00508091. DOI
Lochmuller CH, Wigman LS. Affinity separations in magnetically stabilized fluidized beds. Synthesis and performance of packing materials. Sep Sci Technol. 1987;22:2111–2125.
Lochmuller CH, Wigman LS, Kitchell BS. Aerosol-jet produced, magnetic carrageenan-gel particles: a new affinity chromatography matrix. J Chem Technol Biotechnol. 1987;40:33–40.
Cocker TM, Fee CJ, Evans RA. Preparation of magnetically susceptible polyacrylamide/magnetite beads for use in magnetically stabilized fluidized bed chromatography. Biotechnol Bioeng. 1997;53:79–87. PubMed
An XN, Su ZX. Characterization and application of high magnetic property chitosan particles. J Appl Polym Sci. 2001;81:1175–1181. doi: 10.1002/app.1539. DOI
Nishiya Y, Hibi T, Oda JL. A purification method of the diagnostic enzyme Bacillus uricase using magnetic beads and non-specific protease. Protein Expr Purif. 2002;25:426–429. doi: 10.1016/S1046-5928(02)00022-0. PubMed DOI
Dong YS, Liang F, Jin HX, Xi Q, Chang JH. Preparation of novel magnetic affinity adsorbents and application for purification of urokinase. Chem J Chin Univ – Chin. 2002;23:1013–1017.
Dumitrascu G, Kumbhar A, Zhou WL, Rosenzweig Z. The use of derivatized magnetoliposomes for extraction of antibodies from aqueous solutions. IEEE Trans Magn. 2001;37:2932–2934. doi: 10.1109/20.951351. DOI
Odabasi M, Denizli A. Polyhydroxyethylmethacrylate-based magnetic DNA-affinity beads for anti-DNA antibody removal from systemic lupus erythematosus patient plasma. J Chromatogr B. 2001;760:137–148. PubMed
Li X, Li CX, He BL. Preparation and application of magnetic affinity adsorbent based on magnetic cellulose bead. Chem J Chin Univ – Chin. 1998;19:994–999.
Quitadamo IJ, Kostman TA, Schelling ME, Franceschi VR. Magnetic bead purification as a rapid and efficient method for enhanced antibody specificity for plant sample immunoblotting and immunolocalization. Plant Sci. 2000;153:7–14. doi: 10.1016/S0168-9452(99)00243-5. DOI
Shinkai M, Kamihira M, Honda H, Kobayashi T. Rapid purification of monoclonal antibody with functional magnetite particles. Kag Kog Ronbunshu. 1992;18:256–259.
Kondo A, Kamura H, Higashitani K. Development and application of thermo-sensitive magnetic immunomicrospheres for antibody purification. Appl Microbiol Biotechnol. 1994;41:99–105. doi: 10.1007/s002530050114. PubMed DOI
Ozkara S, Akgol S, Canak Y, Denizli A. A novel magnetic adsorbent for immunoglobulin-G purification in a magnetically stabilized fluidized bed. Biotechnol Progress. 2004;20:1169–1175. doi: 10.1021/bp049896s. PubMed DOI
Meng ZH, Lin B, Xie YH, Zhang KQ. Cloning and expression of fused Fc-binding protein (SPA-SPG) and its application in purification of IgG. Progr Biochem Biophys. 2004;31:146–149.
Quitadamo IJ, Schelling ME. Efficient purification of mouse anti-FGF receptor IgM monoclonal antibody by magnetic beads. Hybridoma. 1998;17:199–207. PubMed
Bhagwati S, Ghatpande A, Leung B. Identification of two nuclear proteins which bind to RNA CUG repeats: Significance for myotonic dystrophy. Biochem Biophys Res Commun. 1996;228:55–62. doi: 10.1006/bbrc.1996.1615. PubMed DOI
Gabrielsen OS, Hornes E, Korsnes L, Ruet A, Oyen TB. Magnetic DNA affinity purification of yeast transcription factor τ – a new purification principle for the ultrarapid isolation of near homogeneous factor. Nucleic Acids Res. 1989;17:6253–6267. PubMed PMC
Gabrielsen OS, Huet J. Magnetic DNA affinity purification of yeast transcription factor. Meth Enzymol. 1993;218:508–525. PubMed
Hollung K, Gabrielsen OS, Jakobsen KS. Enrichment of DNA-binding proteins from crude tissue for electrophoretic mobility shift assay using magnetic phospho cellulose particles. Nucleic Acids Res. 1994;22:3261–3262. PubMed PMC
Risoen PA, Struksnes K, Myrset AH, Gabrielsen OS. One-step magnetic purification of recombinant DNA-binding proteins using magnetizable phosphocellulose. Protein Expr Purif. 1995;6:272–277. doi: 10.1006/prep.1995.1035. PubMed DOI
Zeng GC, Gao LY, Xia T, Tencomnao T, Yu RK. Characterization of the 5'-flanking fragment of the human GM3-synthase gene. Biochim Biophys Acta. 2003;1625:30–35. PubMed
Gershon PD, Moss B. Early transcription factor subunits are encoded by vaccinia virus late genes. Proc Natl Acad Sci USA. 1990;87:4401–4405. PubMed PMC
Ozyhar A, Gries M, Kiltz HH, Pongs O. Magnetic DNA affinity purification of ecdysteroid receptor. J Steroid Biochem Mol Biol. 1992;43:629–634. doi: 10.1016/0960-0760(92)90287-S. PubMed DOI
Kalivoda KA, Braun KW, Vimr ER. Rapid purification of a prokaryotic regulatory protein with μMACS™ Streptavidin MicroBeads. MACS&more. 2003;7:8–9.
Rahat MA, Fry M. Purification and characterization of p27, a protein from hepatocyte chromatin. Evidence suggesting that it binds selectively to guanine-rich single-stranded DNA. FEBS Lett. 1993;334:60–64. doi: 10.1016/0014-5793(93)81680-X. PubMed DOI
Blank M, Weinschenk T, Priemer M, Schluesener H. Systematic evolution of a DNA aptamer binding to rat brain tumor microvessels. Selective targeting of endothelial regulatory protein pigpen. J Biol Chem. 2001;276:16464–16468. doi: 10.1074/jbc.M100347200. PubMed DOI
Albig A. Isolation of mRNA binding proteins using the μMACS streptavidin kit. MACS&more. 2001;5:6–7.
McKay SJ, Cooke H. A protein which specifically binds to single stranded TTAGGGn repeats. Nucleic Acids Res. 1992;20:1387–1391. PubMed PMC
Chakraborty A, White SM, Schaefer TS, Ball ED, Dyer KF, Tweardy DJ. Granulocyte colony-stimulating factor activation of Stat3a and Stat3β in immature normal and leukemic human myeloid cells. Blood. 1996;88:2442–2449. PubMed
Feghali CA, Wright TM. Ligand-dependent and ligand-independent activation of the transcription factor γRf-1 in a cell-free system. Biochem J. 1995;310:461–467. PubMed PMC
Ren LF, Chen H, Sternberg EA. Tethered bandshift assay and affinity purification of a new DNA-binding protein. Biotechniques. 1994;16:852–855. PubMed
Allen TE, Heidmann S, Reed R, Myler PJ, Goringer HU, Stuart KD. Association of guide RNA binding protein gBP21 with active RNA editing complexes in Trypanosoma brucei. Mol Cell Biol. 1998;18:6014–6022. PubMed PMC
Honys D. Isolation of proteins comprising native gene-specific messenger ribonucleoprotein particles using paramagnetic beads. Plant Sci. 2001;161:605–611. doi: 10.1016/S0168-9452(01)00446-0. DOI
Fantappie MR, Correa-Oliveira R, Caride EC, Geraldo EA, Agnew A, Rumjanek FD. Comparison between site-specific DNA binding proteins of male and female Schistosoma mansoni. Comp Biochem Physiol B – Biochem Mol Biol. 1999;124:33–40. doi: 10.1016/S0305-0491(99)00094-2. PubMed DOI
Kelm RJ, Jr, Cogan JG, Elder PK, Strauch AR, Getz MJ. Molecular interactions between single-stranded DNA-binding proteins associated with an essential MCAT element in the mouse smooth muscle alpha-actin promoter. J Biol Chem. 1999;274:14238–14245. doi: 10.1074/jbc.274.20.14238. PubMed DOI
Daniels DA, Chen H, Hicke BJ, Swiderek KM, Gold L. A tenascin-C aptamer identified by tumor cell SELEX: Systematic evolution of ligands by exponential enrichment. Proc Natl Acad Sci USA. 2003;100:15416–15421. doi: 10.1073/pnas.2136683100. PubMed DOI PMC
Skala-Rubinson H, Vinh J, Labas V, Kahn A, Tuy FPD. Novel target sequences for Pax-6 in the brain-specific activating regions of the rat aldolase C gene. J Biol Chem. 2002;277:47190–47196. doi: 10.1074/jbc.M209349200. PubMed DOI
Murphy MB, Fuller ST, Richardson PM, Doyle SA. An improved method for the in vitro evolution of aptamers and applications in protein detection and purification. Nucleic Acids Res. 2003;31:e110. doi: 10.1093/nar/gng110. PubMed DOI PMC
Tong XD, Sun Y. Agar-based magnetic affinity support for protein adsorption. Biotechnol Progr. 2001;17:738–743. doi: 10.1021/bp010054s. PubMed DOI
Xue B, Tong XD, Sun Y. Characterization of PVA-based magnetic affinity support for protein adsorption. Sep Sci Technol. 2001;36:2449–2461. doi: 10.1081/SS-100106102. DOI
Xue B, Sun Y. Fabrication and characterization of a rigid magnetic matrix for protein adsorption. J Chromatogr A. 2002;947:185–193. doi: 10.1016/S0021-9673(01)01602-8. PubMed DOI
Akgol S, Turkmen D, Denizli A. Cu(II)-incorporated, histidine-containing, magnetic-metal-complexing beads as specific sorbents for the metal chelate affinity of albumin. J Appl Polym Sci. 2004;93:2669–2677. doi: 10.1002/app.20757. DOI
Odabasi M, Uzun L, Denizli A. Porous magnetic chelator support for albumin adsorption by immobilized metal affinity separation. J Appl Polym Sci. 2004;93:2501–2510. doi: 10.1002/app.20826. DOI
Odabasi M, Denizli A. Cibacron Blue F3GA-attached magnetic poly(2-hydroxyethyl methacrylate) beads for human serum albumin adsorption. Polym Int. 2004;53:332–338. doi: 10.1002/pi.1305. DOI
Ding XB, Sun ZH, Zhang WC, Peng YX, Wan GX, Jiang YY. Adsorption/desorption of protein on magnetic particles covered by thermosensitive polymers. J Appl Polym Sci. 2000;77:2915–2920.
Ding XB, Sun ZH, Wan GX, Jiang YY. Interactions between thermosensitive magnetic polymer microspheres and proteins. Acta Polym Sinica. 2000. pp. 9–13.
An XN, Su ZX, Zeng HM. Preparation of highly magnetic chitosan particles and their use for affinity purification of enzymes. J Chem Technol Biotechnol. 2003;78:596–560. doi: 10.1002/jctb.820. DOI
Heeboll-Nielsen A, Dalkiaer M, Hubbuch JJ, Thomas ORT. Superparamagnetic adsorbents for high-gradient magnetic fishing of lectins out of legume extracts. Biotechnol Bioeng. 2004;87:311–323. doi: 10.1002/bit.20116. PubMed DOI
Safarikova M, Safarik I. One-step partial purification of Solanum tuberosum tuber lectin using magnetic chitosan particles. Biotechnol Lett. 2000;22:941–945. doi: 10.1023/A:1005698616574. DOI
Xu CJ, Xu KM, Gu HW, Zhong XF, Guo ZH, Zheng RK, Zhang XX, Xu B. Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins. J Am Chem Soc. 2004;126:3392–3393. doi: 10.1021/ja031776d. PubMed DOI
Muller-Schulte D, Brunner H. Novel magnetic microspheres on the basis of poly(vinyl alcohol) as affinity medium for quantitative detection of glycated hemoglobin. J Chromatogr A. 1995;711:53–60. doi: 10.1016/0021-9673(95)00114-3. PubMed DOI
Dryden SC, Nahhas FA, Nowak JE, Goustin A-S, Tainsky MA. Role for human SIRT2 NAD-dependent deacetylase activity in control of mitotic exit in the cell cycle. Mol Cell Biol. 2003;23:3173–3185. doi: 10.1128/MCB.23.9.3173-3185.2003. PubMed DOI PMC
Ohtsuki T, Sakurai M, Sato A, Watanabe K. Characterization of the interaction between the nucleotide exchange factor EF-Ts from nematode mitochondria and elongation factor Tu. Nucleic Acids Res. 2002;30:5444–5451. doi: 10.1093/nar/gkf679. PubMed DOI PMC
Suzuki M, Kamihira M, Shiraishi T, Takeuchi H, Kobayashi T. Affinity partitioning of protein A using a magnetic aqueous two-phase system. J Ferment Bioeng. 1995;80:78–84. doi: 10.1016/0922-338X(95)98180-S. DOI
Liabakk NB, Nustad K, Espevik T. A rapid and sensitive immunoassay for tumor necrosis factor using magnetic monodisperse polymer particles. J Immunol Methods. 1990;134:253–259. doi: 10.1016/0022-1759(90)90387-B. PubMed DOI
Zhang ZR, O'Sullivan DA, Lyddiatt A. Magnetically stabilised fluidised bed adsorption: practical benefit of uncoupling bed expansion from fluid velocities in the purification of a recombinant protein from Escherichia coli. J Chem Technol Biotechnol. 1999;74:270–274. doi: 10.1002/(SICI)1097-4660(199903)74:3<270::AID-JCTB24>3.0.CO;2-X. DOI
Simonsen A, Stang E, Bremnes B, Roe M, Prydz K, Bakke O. Sorting of MHC class II molecules and the associated invariant chain (Ii) in polarized MDCK cells. J Cell Sci. 1997;110:597–609. PubMed
Mikaelsdottir EK, Valgeirsdottir S, Eyfjord JE, Rafnar T. The Icelandic founder mutation BRCA2 999del5: analysis of expression. Breast Cancer Res. 2004;6:R284–R290. doi: 10.1186/bcr785. PubMed DOI PMC
Welter BH, Price TM. Magnetic separation to concentrate the estrogen receptor from adipose tissue for western analysis. Biotechniques. 1999;27:282–286. PubMed
Chen-Cleland TA, Boffa LC, Carpaneto EM, Mariani MR, Valentin E, Mendez E, Allfrey VG. Recovery of transcriptionally active chromatin restriction fragments by binding to organomercurial-agarose magnetic beads. A rapid and sensitive method for monitoring changes in higher order chromatin structure during gene activation and repression. J Biol Chem. 1993;268:23409–23416. PubMed
Knutson VP, Donnelly PV, Balba Y, Lopez-Reyes M. Insulin resistance is mediated by a proteolytic fragment of the insulin receptor. J Biol Chem. 1995;270:24972–24981. doi: 10.1074/jbc.270.42.24972. PubMed DOI
Chakraborty A, Dyer KF, Cascio M, Mietzner TA, Tweardy DJ. Identification of a novel Stat3 recruitment and activation motif within the granulocyte colony-stimulating factor receptor. Blood. 1999;93:15–24. PubMed
Tanaka T, Matsunaga T. Detection of HbA(1c) by boronate affinity immunoassay using bacterial magnetic particles. Biosens Bioelectron. 2001;16:1089–1094. doi: 10.1016/S0956-5663(01)00187-7. PubMed DOI
Karlsson GB, Platt FM. Analysis and isolation of human transferrin receptor using the OKT-9 monoclonal antibody covalently cross-linked to magnetic beads. Anal Biochem. 1991;199:219–222. doi: 10.1016/0003-2697(91)90093-9. PubMed DOI
Apoga D, Ek B, Tunlid A. Analysis of proteins in the extracellular matrix of the plant pathogenic fungus Bipolaris sorokiniana using 2-D gel electrophoresis and MS/MS. FEMS Microbiol Lett. 2001;197:145–150. doi: 10.1016/S0378-1097(01)00052-0. PubMed DOI
Hincha DK, Neukamm B, Sror HAM, Sieg F, Weckwarth W, Ruckels M, Lullien-Pellerin V, Schroder W, Schmitt JM. Cabbage cryoprotectin is a member of the nonspecific plant lipid transfer protein gene family. Plant Physiol. 2001;125:835–846. doi: 10.1104/pp.125.2.835. PubMed DOI PMC
Peter J, Unverzagt C, Lenz H, Hoesel W. Purification of prostate-specific antigen from human serum by indirect immunosorption and elution with a hapten. Anal Biochem. 1999;273:98–104. doi: 10.1006/abio.1999.4215. PubMed DOI
Peter J, Unverzagt C, Krogh TN, Vorm O, Hoesel W. Identification of precursor forms of free prostate-specific antigen in serum of prostate cancer patients by immunosorption and mass spectrometry. Cancer Res. 2001;61:957–962. PubMed
Yang Y-S, Yang M-CW, Wang B, Weissler JC. BR22, a novel protein, interacts with thyroid transcription factor-1 and activates the human surfactant protein B promoter. Am J Respir Cell Mol Biol. 2001;24:30–37. PubMed
Persaud DR, Yousefi V, Haunerland N. Efficient isolation, purification, and characterization of the Helicoverpa zea VHDL receptor. Protein Expr Purif. 2003;32:260–264. doi: 10.1016/S1046-5928(03)00236-5. PubMed DOI
Salazar R, Brandt R, Kellermann J, Krantz S. Purification and characterization of a 200 kDa fructosyllysine-specific binding protein from cell membranes of U937 cells. Glycoconjugate J. 2000;17:713–716. doi: 10.1023/A:1011074705615. PubMed DOI
Girault S, Chassaing G, Blais JC, Brunot A, Bolbach G. Coupling of MALDI-TOF mass analysis to the separation of biotinylated peptides by magnetic streptavidin beads. Anal Chem. 1996;68:2122–2126. doi: 10.1021/ac960043r. PubMed DOI
Ji Z, Pinon DI, Miller LJ. Development of magnetic beads for rapid and efficient metal-chelate affinity purifications. Anal Biochem. 1996;240:197–201. doi: 10.1006/abio.1996.0349. PubMed DOI
Samson I, Rozenski J, Vanaerschot A, Samyn B, Vanbeeumen J, Herdewijn P. Screening of a synthetic pentapeptide library composed of D-amino acids against fructose-1,6-biphosphate aldolase. Lett Pept Sci. 1995;2:259–260. doi: 10.1007/BF00119166. DOI
Seino S, Kinoshita T, Otome Y, Nakagawa T, Okitsu K, Nakayama T, Sekino T, Niihara K, Yamamoto TA. Radiation-induced synthesis of Au/Fe oxide nanocomposite particles for magnetic separation of biomolecules. J Ceram Process Res. 2004;5:136–139.
Prioult G, Turcotte C, Labarre L, Lacroix C, Fliss I. Rapid purification of nisin Z using specific monoclonal antibody-coated magnetic beads. Int Dairy J. 2000;10:627–633. doi: 10.1016/S0958-6946(00)00093-5. DOI
Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings