Magnetic alginate microparticles for purification of alpha-amylases
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
14580797
DOI
10.1016/j.jbiotec.2003.07.002
PII: S0168165603002074
Knihovny.cz E-zdroje
- MeSH
- alfa-amylasy chemie izolace a purifikace MeSH
- algináty chemie MeSH
- koncentrace vodíkových iontů MeSH
- magnetismus * MeSH
- mikrosféry MeSH
- mikroskopie elektronová rastrovací MeSH
- vazba proteinů MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- alfa-amylasy MeSH
- algináty MeSH
Spherical magnetic alginate microparticles (25-60 microm in diameter) were prepared using the microemulsion system, with water-saturated 1-pentanol as the organic phase. The limited solubility of 1-pentanol in water enabled simple removal of the organic solvent from the prepared beads with water solution. The prepared alginate microparticles were used as magnetic affinity adsorbents for specific purification of alpha-amylases. Enzyme activity was eluted by 1.0 M maltose. alpha-Amylases from Bacillus amyloliquefaciens and porcine pancreatic acetone powder were purified 9- and 12-fold with 88 and 96% activity recovery, respectively.
Citace poskytuje Crossref.org
Magnetic techniques for the isolation and purification of proteins and peptides
