Magnetic alginate microparticles for purification of alpha-amylases
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
14580797
DOI
10.1016/j.jbiotec.2003.07.002
PII: S0168165603002074
Knihovny.cz E-resources
- MeSH
- alpha-Amylases chemistry isolation & purification MeSH
- Alginates chemistry MeSH
- Hydrogen-Ion Concentration MeSH
- Magnetics * MeSH
- Microspheres MeSH
- Microscopy, Electron, Scanning MeSH
- Protein Binding MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- alpha-Amylases MeSH
- Alginates MeSH
Spherical magnetic alginate microparticles (25-60 microm in diameter) were prepared using the microemulsion system, with water-saturated 1-pentanol as the organic phase. The limited solubility of 1-pentanol in water enabled simple removal of the organic solvent from the prepared beads with water solution. The prepared alginate microparticles were used as magnetic affinity adsorbents for specific purification of alpha-amylases. Enzyme activity was eluted by 1.0 M maltose. alpha-Amylases from Bacillus amyloliquefaciens and porcine pancreatic acetone powder were purified 9- and 12-fold with 88 and 96% activity recovery, respectively.
References provided by Crossref.org
Magnetic techniques for the isolation and purification of proteins and peptides
