Localization of self-interacting domains within betaretrovirus Gag polyproteins
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, P.H.S.
Grantová podpora
R01 AI043230-05
NIAID NIH HHS - United States
R01AI43230
NIAID NIH HHS - United States
PubMed
15680431
DOI
10.1016/j.virol.2004.12.007
PII: S0042-6822(04)00838-4
Knihovny.cz E-zdroje
- MeSH
- DNA primery MeSH
- Escherichia coli genetika MeSH
- genové produkty gag chemie genetika metabolismus MeSH
- kapsida MeSH
- Masonův-Pfizerův opičí virus metabolismus MeSH
- molekulární sekvence - údaje MeSH
- plazmidy MeSH
- polymerázová řetězová reakce MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- vazebná místa MeSH
- virus myšího tumoru prsní žlázy genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Názvy látek
- DNA primery MeSH
- genové produkty gag MeSH
The Betaretrovirus genus is characterized by the ability to preassemble immature capsids within the cytoplasm. For Mason-Pfizer monkey virus (M-PMV) this ability depends in part upon the unique Internal Scaffold Domain (ISD) within the p12 region of Gag. In this study, we have further characterized the ability of M-PMV p12 to promote Gag-Gag interaction and have examined the Gag polyprotein of the related mouse mammary tumor virus (MMTV) to potentially identify a region with equivalent function. Using the yeast two-hybrid system, we confirmed that both Gag polyproteins strongly interact, primarily through the CA-NC regions, but also through additional domains N-terminal to CA. For M-PMV, this auxiliary interaction domain was p12. For MMTV, no single strongly self-interacting protein was identified. Instead, MMTV Gag appears to utilize the weak contributions of several protein domains to support the main interaction of its CA-NC. Our findings suggest that, in addition to the canonical NC "I-domain" interaction, MMTV Gag self-association results from the concerted action of multiple regions of the polyprotein while M-PMV Gag relies mainly on its p12 domain.
Citace poskytuje Crossref.org