Localization of self-interacting domains within betaretrovirus Gag polyproteins
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, P.H.S.
Grantová podpora
R01 AI043230
NIAID NIH HHS - United States
R01AI43230
NIAID NIH HHS - United States
PubMed
15680431
DOI
10.1016/j.virol.2004.12.007
PII: S0042-6822(04)00838-4
Knihovny.cz E-zdroje
- MeSH
- DNA primery MeSH
- Escherichia coli genetika MeSH
- genové produkty gag chemie genetika metabolismus MeSH
- kapsida MeSH
- Masonův-Pfizerův opičí virus metabolismus MeSH
- molekulární sekvence - údaje MeSH
- plazmidy MeSH
- polymerázová řetězová reakce MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- vazebná místa MeSH
- virus myšího tumoru prsní žlázy genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Názvy látek
- DNA primery MeSH
- genové produkty gag MeSH
The Betaretrovirus genus is characterized by the ability to preassemble immature capsids within the cytoplasm. For Mason-Pfizer monkey virus (M-PMV) this ability depends in part upon the unique Internal Scaffold Domain (ISD) within the p12 region of Gag. In this study, we have further characterized the ability of M-PMV p12 to promote Gag-Gag interaction and have examined the Gag polyprotein of the related mouse mammary tumor virus (MMTV) to potentially identify a region with equivalent function. Using the yeast two-hybrid system, we confirmed that both Gag polyproteins strongly interact, primarily through the CA-NC regions, but also through additional domains N-terminal to CA. For M-PMV, this auxiliary interaction domain was p12. For MMTV, no single strongly self-interacting protein was identified. Instead, MMTV Gag appears to utilize the weak contributions of several protein domains to support the main interaction of its CA-NC. Our findings suggest that, in addition to the canonical NC "I-domain" interaction, MMTV Gag self-association results from the concerted action of multiple regions of the polyprotein while M-PMV Gag relies mainly on its p12 domain.
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