Development of an indirect competitive ELISA for detection of Campylobacter jejuni subsp.jejuni O:23 in foods
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
15702549
DOI
10.1007/bf02931537
Knihovny.cz E-zdroje
- MeSH
- Campylobacter jejuni imunologie izolace a purifikace patogenita MeSH
- ELISA metody statistika a číselné údaje MeSH
- enteritida mikrobiologie MeSH
- kampylobakterové infekce mikrobiologie MeSH
- králíci MeSH
- kur domácí MeSH
- lidé MeSH
- potravinářská mikrobiologie * MeSH
- protilátky bakteriální MeSH
- senzitivita a specificita MeSH
- zkřížené reakce MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- protilátky bakteriální MeSH
An indirect enzyme immunoassay for rapid detection of Campylobacter jejuni subsp. jejuni 0:23 has been developed. Optimum concentrations of immobilized cells, polyclonal chicken IgY, and rabbit anti-IgY antibody-horseradish peroxidase conjugate were 3.1 CFU/nL, 10 microg/mL, and 8 microg/mL, respectively. Under such conditions, the detection limit reached 50 CFU/microL, limit of quantification being 480 CFU/microL. By testing 5 chromogens, viz. 1,2-benzenediamine, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid), 3,3',5,5'-tetramethylbenzidine, bi(4,4'-anisidine) and 3-methyl-2-benzothiazolinone hydrazone, in horseradish peroxidase substrate, 1,2-benzenediamine or 3,3',5,5'-tetramethylbenzidine as H-donors in the enzyme substrate provided the highest ELISA sensitivity. The applied polyclonal antibody was specific for homogeneous antigen. The cross-reactions were observed only with one strain of C. sputorum subsp. sputorum (21.5 %) and with G+ bacterium Micrococcus luteus (6.1 %). Preliminary tests have been performed with a limited number of artificially contaminated food samples. No matrix effects on the ELISA sensitivity were observed. The results (by means of ELISA) were comparable with those given by both a standard cultivation method performed according to CSN ISO 10272 and commercially available Singlepath Campylobacter GLISA-Rapid Test.
Department of Biochemistry and Microbiology Institute of Chemical Technology 166 28 Prague 6 Czechia
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Appl Environ Microbiol. 1993 Jul;59(7):2161-5 PubMed
Appl Environ Microbiol. 1997 Nov;63(11):4558-63 PubMed
Am Biotechnol Lab. 1994 Nov;12(12):44-5 PubMed
Br Med J (Clin Res Ed). 1981 Apr 25;282(6273):1374-6 PubMed
Lett Appl Microbiol. 2000 Sep;31(3):209-12 PubMed
J Clin Microbiol. 1991 Oct;29(10):2093-8 PubMed
Lett Appl Microbiol. 1996 Apr;22(4):288-92 PubMed
Arch Virol. 1982;74(4):277-91 PubMed
J Clin Chem Clin Biochem. 1985 Jan;23(1):41-4 PubMed
J Clin Microbiol. 1984 Feb;19(2):169-71 PubMed
Poult Sci. 1997 Feb;76(2):314-7 PubMed
Br Med J. 1977 Jul 2;2(6078):9-11 PubMed
Epidemiol Infect. 1993 Jun;110(3):601-7 PubMed
Lancet. 1981 Aug 15;2(8242):352-4 PubMed
J Clin Microbiol. 1982 May;15(5):761-8 PubMed
Lancet. 1980 Mar 29;1(8170):713-4 PubMed
Can J Microbiol. 1981 Aug;27(8):841-2 PubMed
Biometrics. 1990 Jun;46(2):381-90 PubMed
J Food Prot. 1995 Mar;58(3):326-344 PubMed
J Clin Microbiol. 1986 Mar;23(3):456-9 PubMed
J Appl Bacteriol. 1986 Aug;61(2):125-32 PubMed
Lancet. 1978 Nov 4;2(8097):979-81 PubMed
Int J Food Microbiol. 1997 Jun 17;37(1):87-91 PubMed
J Clin Microbiol. 1988 Jan;26(1):47-9 PubMed
Epidemiol Infect. 2000 Oct;125(2):269-75 PubMed
Eur J Clin Microbiol. 1983 Aug;2(4):378-83 PubMed
FEMS Immunol Med Microbiol. 1997 Sep;19(1):47-56 PubMed
Lett Appl Microbiol. 1993 Nov;17(5):235-7 PubMed
Vaccine. 1997 Dec;15(17-18):1922-32 PubMed
Lancet. 1980 May 31;1(8179):1188 PubMed
Int J Food Microbiol. 2001 Aug 15;68(1-2):53-9 PubMed
J Pediatr. 1981 Feb;98(2):254-7 PubMed
J Clin Microbiol. 1980 Dec;12(6):732-7 PubMed
J Appl Bacteriol. 1989 Feb;66(2):127-35 PubMed
J Appl Bacteriol. 1996 Nov;81(5):467-73 PubMed
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