Characterization of rabbit antibodies for immunochemical detection of Yersinia enterocolitica
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
18298049
DOI
10.1007/bf02932112
Knihovny.cz E-zdroje
- MeSH
- antigeny bakteriální imunologie MeSH
- antisérum biosyntéza MeSH
- barvení stříbrem MeSH
- ELISA ekonomika metody MeSH
- endopeptidasa K metabolismus MeSH
- imunizace MeSH
- imunoblotting MeSH
- imunoglobulin G imunologie izolace a purifikace MeSH
- imunologické techniky MeSH
- králíci MeSH
- lipopolysacharidy analýza MeSH
- protilátky bakteriální analýza imunologie MeSH
- specificita protilátek * MeSH
- Yersinia enterocolitica chemie imunologie izolace a purifikace MeSH
- Yersinia imunologie izolace a purifikace MeSH
- zkřížené reakce MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antigeny bakteriální MeSH
- antisérum MeSH
- endopeptidasa K MeSH
- imunoglobulin G MeSH
- lipopolysacharidy MeSH
- protilátky bakteriální MeSH
Rabbit IgG raised against whole cells of Yersinia enterocolitica O:3, O:9 and against a group of pathogenic Y. enterocolitica strains (serotypes O:3, O:5,27, O:8. and O:9) were prepared. The antibody limiting titers were within the range of 1:9.5 x 10(4)-1:7.5 x 10(5). The immunoblotting analysis of Yersinia lipopolysacchides separated by SDS-PAGE showed that IgG against the single serotype O:3 interacted with high-molar-mass LPS of O:3 whereas other antibodies were bound to low-molar-mass LPS of serotypes O:3, O:5,27, O:9 and strain Y. enterocolitica (CNCTC Y 2/68). IgG against the group of pathogenic serotypes also weakly interacted with low-molar-mass LPS of serotypes O:5, O:6,30, and O:10. The cross-reactivity of the antibodies with Y. pseudotuberculosis Ia and/or Y. rohdei b, d, e, f, i, which was observed by means of dot-blotting procedure using the whole bacterial cells as an antigen, was shown not to be caused by LPS of these bacteria. The prepared antibodies were used in the development of indirect competitive ELISA. At the optimum concentration of the immunoreactants the detection limits were within the range of 3-7 x 10(6) colony-forming units per mL.
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