Characterization of rabbit antibodies for immunochemical detection of Yersinia enterocolitica
Language English Country United States Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
18298049
DOI
10.1007/bf02932112
Knihovny.cz E-resources
- MeSH
- Antigens, Bacterial immunology MeSH
- Immune Sera biosynthesis MeSH
- Silver Staining MeSH
- Enzyme-Linked Immunosorbent Assay economics methods MeSH
- Endopeptidase K metabolism MeSH
- Immunization MeSH
- Immunoblotting MeSH
- Immunoglobulin G immunology isolation & purification MeSH
- Immunologic Techniques MeSH
- Rabbits MeSH
- Lipopolysaccharides analysis MeSH
- Antibodies, Bacterial analysis immunology MeSH
- Antibody Specificity * MeSH
- Yersinia enterocolitica chemistry immunology isolation & purification MeSH
- Yersinia immunology isolation & purification MeSH
- Cross Reactions MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, Bacterial MeSH
- Immune Sera MeSH
- Endopeptidase K MeSH
- Immunoglobulin G MeSH
- Lipopolysaccharides MeSH
- Antibodies, Bacterial MeSH
Rabbit IgG raised against whole cells of Yersinia enterocolitica O:3, O:9 and against a group of pathogenic Y. enterocolitica strains (serotypes O:3, O:5,27, O:8. and O:9) were prepared. The antibody limiting titers were within the range of 1:9.5 x 10(4)-1:7.5 x 10(5). The immunoblotting analysis of Yersinia lipopolysacchides separated by SDS-PAGE showed that IgG against the single serotype O:3 interacted with high-molar-mass LPS of O:3 whereas other antibodies were bound to low-molar-mass LPS of serotypes O:3, O:5,27, O:9 and strain Y. enterocolitica (CNCTC Y 2/68). IgG against the group of pathogenic serotypes also weakly interacted with low-molar-mass LPS of serotypes O:5, O:6,30, and O:10. The cross-reactivity of the antibodies with Y. pseudotuberculosis Ia and/or Y. rohdei b, d, e, f, i, which was observed by means of dot-blotting procedure using the whole bacterial cells as an antigen, was shown not to be caused by LPS of these bacteria. The prepared antibodies were used in the development of indirect competitive ELISA. At the optimum concentration of the immunoreactants the detection limits were within the range of 3-7 x 10(6) colony-forming units per mL.
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