Susceptibility of IFN-gamma or IL-12 knock-out and SCID mice to infection with two microsporidian species, Encephalitozoon cuniculi and E. intestinalis
Language English Country Czech Republic Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
15729937
Knihovny.cz E-resources
- MeSH
- CD4-Positive T-Lymphocytes immunology MeSH
- CD8-Positive T-Lymphocytes immunology MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Encephalitozoon immunology MeSH
- Encephalitozoonosis immunology parasitology MeSH
- Interferon-gamma deficiency immunology MeSH
- Interleukin-12 deficiency immunology MeSH
- Mice, Inbred BALB C MeSH
- Mice, Inbred C57BL MeSH
- Mice, Knockout MeSH
- Mice, SCID MeSH
- Mice MeSH
- Adoptive Transfer MeSH
- Antibodies, Protozoan blood MeSH
- Flow Cytometry MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Interferon-gamma MeSH
- Interleukin-12 MeSH
- Antibodies, Protozoan MeSH
Susceptibility of three strains of immunodeficient mice to two related microsporidian species Encephalitozoon cuniculi Levaditi, Nicolau et Schoen, 1923 and Encephalitozoon intestinalis (Cali, Kotler et Orenstein, 1993) was compared. While both, severe combined immunodeficient (SCID) and interferon-gamma knock-out (IFN-gamma KO) mice, succumbed to either intraperitoneal (i.p.) or peroral (p.o.) (natural) infection with both parasites, only i.p. infection with E. cuniculi killed interleukin-12 knock-out (IL-12 KO) mice. IFN-gamma KO mice died earlier than SCID mice. Adoptive transfer of naive splenocytes from IFN-gamma KO mice did not protect the SCID mice from a lethal infection with either of the Encephalitozoon species. However, reconstituted mice survived significantly longer (P<0.05), thus indicating the role of IFN-gamma produced by host NK cells in the development of mechanisms of anti-microsporidial protective immunity. Non-lethal outcome of the infection always correlated with the increase in CD8+ T lymphocyte subpopulation. Both E. intestinalis-infected IFN-gamma KO and IL-12 KO mice produced comparable levels of specific antibodies, suggesting that antibodies did not protect IFN-gamma KO mice from lethal infection.