Magnetic hydrophilic methacrylate-based polymer microspheres for genomic DNA isolation
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15739893
DOI
10.1016/j.chroma.2004.12.014
PII: S0021-9673(04)02255-1
Knihovny.cz E-resources
- MeSH
- DNA genetics isolation & purification MeSH
- Electrophoresis, Agar Gel MeSH
- Genome * MeSH
- Magnetics * MeSH
- Methacrylates chemistry MeSH
- Microspheres MeSH
- Microscopy, Electron, Scanning MeSH
- Polymerase Chain Reaction MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA MeSH
- Methacrylates MeSH
Carboxyl groups containing magnetic and non-magnetic microspheres were used in solid-phase reversible immobilization (SPRI) of genomic DNA. Magnetic non-porous poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate)--P(HEMA-co-EDMA), poly(glycidyl methacrylate)--PGMA and P(HEMA-co-GMA) microspheres with hydrophilic properties were prepared by dispersion copolymerization of the respective monomers in the presence of colloidal iron oxides. DNA from chicken erythrocytes and DNA isolated from bacterial cells of Bifidobacterium longum was used for testing of adsorption/desorption properties of magnetic microspheres. The occurrence of false negative results in polymerase chain reaction (PCR) caused by the presence of extracellular inhibitors in DNA samples has been solved using SPRI. The P(HEMA-co-EDMA) and P(HEMA-co-GMA) microspheres were used for isolation of DNA from different dairy products followed by PCR identification of Bifidobacterium strains.
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