Immune response after adjuvant mucosal immunization of mice with inactivated influenza virus
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15752565
DOI
10.1016/j.imlet.2004.10.023
PII: S0165-2478(04)00298-6
Knihovny.cz E-resources
- MeSH
- Adjuvants, Immunologic * MeSH
- Lymphocyte Activation MeSH
- Betainfluenzavirus immunology MeSH
- Cell Line MeSH
- Cytokines biosynthesis genetics immunology MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Virus Inactivation * MeSH
- Vaccines, Inactivated immunology MeSH
- Macrophages immunology metabolism MeSH
- RNA, Messenger genetics metabolism MeSH
- Mice MeSH
- Neutralization Tests MeSH
- Lung immunology metabolism pathology MeSH
- Antibodies, Viral analysis immunology MeSH
- Dogs MeSH
- Immunity, Mucosal immunology MeSH
- T-Lymphocytes immunology MeSH
- Influenza Vaccines immunology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Dogs MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adjuvants, Immunologic * MeSH
- Cytokines MeSH
- Vaccines, Inactivated MeSH
- RNA, Messenger MeSH
- Antibodies, Viral MeSH
- Influenza Vaccines MeSH
Satisfactory mucosal immunity in the respiratory tract is very important for protection against influenza. It can be achieved only by mucosal immunization. Mucosal vaccination with inactivated influenza virus may not be sufficiently effective and suitable adjuvants are therefore sought. We tested intratracheal immunization of mice with inactivate B type influenza virus in a mixture with formolized G+ bacterium Bacillus firmus, whose adjuvant effects have previously been documented in another system. The treatment resulted in a marked increase of both systemic and mucosal antibody response in IgG and IgA classes. Stimulation of T lymphocytes after adjuvant immunization was very mild, no proliferation taking place after specific stimulation with antigen in vitro. However, slightly increased systemic (spleen) and local (lungs) production of cytokines without perceptible Th1/Th2 polarization was determined. B. firmus is an efficient adjuvant in respiratory tract immunization while with subcutaneous immunization it lowers the antibody response.
References provided by Crossref.org
Immunomodulatory effects of Bacillus firmus on mouse peritoneal cells in vitro
