Miniaturized kinetic growth inhibition assay with denitrifying bacteria Paracoccus denitrificans
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15910901
DOI
10.1016/j.chemosphere.2005.01.007
PII: S0045-6535(05)00104-9
Knihovny.cz E-resources
- MeSH
- Agrochemicals toxicity MeSH
- Biological Assay MeSH
- Nitrogen metabolism MeSH
- Kinetics MeSH
- Culture Media MeSH
- Paracoccus drug effects growth & development metabolism MeSH
- Pesticides toxicity MeSH
- Pseudomonas drug effects growth & development metabolism MeSH
- Metals, Heavy toxicity MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Agrochemicals MeSH
- Nitrogen MeSH
- Culture Media MeSH
- Pesticides MeSH
- Metals, Heavy MeSH
A method to cultivate anaerobic bacteria on standard 96-well microplates with automatic recording of growth curves is presented. The method was used as a kinetic growth inhibition assay with denitrifying bacteria Paracoccus denitrificans, and applied to various heavy metal ions and selected agrochemicals. Incorporated in a battery of other biotest the assay could take into account effects of toxicants on denitrifying organisms. The results (EC50) revealed that the assay was relatively sensitive. Performed in vials, the assay was also applied to toxicity testing of volatile compounds and represented a convenient method for assessing samples containing volatile constituents.
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