Oligonucleotide-based microarray: a new improvement in microarray detection of plant viruses
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
15927276
DOI
10.1016/j.jviromet.2005.04.009
PII: S0166-0934(05)00134-5
Knihovny.cz E-zdroje
- MeSH
- druhová specificita MeSH
- nemoci rostlin virologie MeSH
- oligonukleotidové sondy * MeSH
- oligonukleotidy * chemická syntéza chemie genetika MeSH
- rostlinné viry klasifikace genetika izolace a purifikace MeSH
- sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů metody MeSH
- senzitivita a specificita MeSH
- Solanum tuberosum virologie MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- oligonukleotidové sondy * MeSH
- oligonukleotidy * MeSH
Microarrays are one of the new emerging methods in plant virology currently being developed by various laboratories. In this study, a new approach is described on the detection of plant viruses using short synthetic single-stranded oligomers (40 nt) instead of PCR products as capture probes. A microchip detecting potato viruses, PVA, PVS, PVM, PVX, PVY and PLRV, in both single and mixed infections was developed and tested. The chip was also designed to distinguish between the main strains of PVY and PVS. Results of initial tests with PVY(NTN) and PVY(O) strains using several different probes for one virus are presented. Possibilities and advantages of the new oligonucleotide-based microarray approach for plant viral diagnosis are discussed.
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