Evaluation of (GTG)5-PCR for identification of Enterococcus spp
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
15927748
DOI
10.1016/j.femsle.2005.04.030
PII: S0378-1097(05)00262-4
Knihovny.cz E-zdroje
- MeSH
- DNA bakterií MeSH
- DNA fingerprinting MeSH
- DNA primery * MeSH
- Enterococcus klasifikace genetika MeSH
- polymerázová řetězová reakce metody normy MeSH
- potravinářská mikrobiologie MeSH
- reprodukovatelnost výsledků MeSH
- techniky typizace bakterií MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA bakterií MeSH
- DNA primery * MeSH
A set of reference strains and a group of previously unidentified enterococci were analysed by rep-PCR with the (GTG)(5) primer to evaluate the discriminatory power and suitability of this method for typing and identification of enterococcal species. A total of 49 strains representing all validly described species were obtained from bacterial collections. For more extensive evaluation of this identification approach 112 well-defined and identified enterococci isolated from bryndza cheese were tested. The (GTG)(5)-PCR fingerprinting assigned all strains into well-differentiated clusters representing individual species. Subsequently, a group including 44 unidentified enterococci isolated from surface waters was analysed to evaluate this method for identification of unknown isolates. Obtained band patterns allowed us to identify all the strains clearly to the species level. This study proved that rep-PCR with (GTG)(5) primer is a reliable and fast method for species identification of enterococci.
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