Corneal rat-to-mouse xenotransplantation and the effects of anti-CD4 or anti-CD8 treatment on cytokine and nitric oxide production
Language English Country Switzerland Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15948866
DOI
10.1111/j.1432-2277.2005.00112.x
PII: TRI112
Knihovny.cz E-resources
- MeSH
- CD4 Antigens immunology MeSH
- CD8 Antigens immunology MeSH
- Cytokines biosynthesis MeSH
- Gene Expression MeSH
- Rats MeSH
- Antibodies, Monoclonal pharmacology MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Nitric Oxide biosynthesis MeSH
- Rats, Inbred Lew MeSH
- Graft Survival drug effects MeSH
- Cornea metabolism MeSH
- Nitric Oxide Synthase Type II MeSH
- Nitric Oxide Synthase genetics metabolism MeSH
- Transplantation, Heterologous * MeSH
- Corneal Transplantation * MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- CD4 Antigens MeSH
- CD8 Antigens MeSH
- Cytokines MeSH
- Antibodies, Monoclonal MeSH
- Nos2 protein, mouse MeSH Browser
- Nos2 protein, rat MeSH Browser
- Nitric Oxide MeSH
- Nitric Oxide Synthase Type II MeSH
- Nitric Oxide Synthase MeSH
Corneal xenotransplantation may be an alternative approach to overcome shortage of allografts for clinical transplantation. Orthotopic corneal rat-to-mouse xenotransplantation and syngeneic transplantation was performed and the effects of anti-CD4 and anti-CD8 treatments on corneal xenograft survival and production of cytokines, interleukin (IL)-2, IL-4, IL-10, gamma-interferon (IFN-gamma) and nitric oxide (NO) were evaluated. RT-PCR was used to determine the expression of genes for cytokines and inducible nitric oxide synthase (iNOS) in the grafts. The presence of iNOS protein in grafts was detected by immunofluorescent staining. We found that corneal xenotransplantation was associated with a strong upregulation of genes for both Th1 and Th2 cytokines and with NO production in the graft. Treatment of xenograft recipients with mAb anti-CD4, but not anti-CD8, resulted in a profound inhibition of IL-2, IL-4 and IL-10 production, and in a significant prolongation of corneal xenograft survival. The results show that upregulation of Th2 cytokines after corneal xenotransplantation does not correlate with xenograft rejection. Rather, corneal graft rejection is associated with the expression of genes for IFN-gamma and iNOS and with NO production.
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