Lipopolysaccharide-mediated protection against Klebsiella pneumoniae-induced lobar pneumonia: intranasal vs. intramuscular route of immunization
Language English Country United States Media print
Document type Journal Article
PubMed
15954538
DOI
10.1007/bf02931298
Knihovny.cz E-resources
- MeSH
- Administration, Intranasal MeSH
- Pneumonia, Bacterial microbiology prevention & control MeSH
- Immunization * MeSH
- Klebsiella Infections microbiology prevention & control MeSH
- Injections, Intramuscular MeSH
- Klebsiella pneumoniae growth & development immunology MeSH
- Lipopolysaccharides administration & dosage immunology isolation & purification MeSH
- Disease Models, Animal MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Lung microbiology pathology MeSH
- Colony Count, Microbial MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- India MeSH
- Names of Substances
- Lipopolysaccharides MeSH
Immunoprotective potential of delivered lipopolysaccharide (LPS) preparation from Klebsiella pneumoniae was determined in a murine model of lobar pneumonia. Protection was assessed with three doses of LPS (25, 50 and 100 microg; without any adjuvant) administered intranasally or intramuscularly. After evaluation of lung tissue (bacterial load and histopathology), no significant protection was observed at 25 microg with either application. A significant decrease in lung bacterial load coupled with fall in severity of lung lesions was observed with 50 microg (again both applications). At 100 microg dose, with intramuscular route, a further decrease in the lung bacterial load was shown compared to the 50 microg dose. In contrast, 100 microg LPS, when given intranasally, resulted in a higher bacterial colonization of the lung tissue and higher lung pathology; thus we recommend intramuscular instead of the intranasal route for developing protection against K. pneumoniae-mediated pneumonia with intact LPS-based vaccines.
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