Lipopolysaccharide-mediated protection against Klebsiella pneumoniae-induced lobar pneumonia: intranasal vs. intramuscular route of immunization
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
15954538
DOI
10.1007/bf02931298
Knihovny.cz E-zdroje
- MeSH
- aplikace intranazální MeSH
- bakteriální pneumonie mikrobiologie prevence a kontrola MeSH
- imunizace * MeSH
- infekce bakteriemi rodu Klebsiella mikrobiologie prevence a kontrola MeSH
- injekce intramuskulární MeSH
- Klebsiella pneumoniae růst a vývoj imunologie MeSH
- lipopolysacharidy aplikace a dávkování imunologie izolace a purifikace MeSH
- modely nemocí na zvířatech MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- plíce mikrobiologie patologie MeSH
- počet mikrobiálních kolonií MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Indie MeSH
- Názvy látek
- lipopolysacharidy MeSH
Immunoprotective potential of delivered lipopolysaccharide (LPS) preparation from Klebsiella pneumoniae was determined in a murine model of lobar pneumonia. Protection was assessed with three doses of LPS (25, 50 and 100 microg; without any adjuvant) administered intranasally or intramuscularly. After evaluation of lung tissue (bacterial load and histopathology), no significant protection was observed at 25 microg with either application. A significant decrease in lung bacterial load coupled with fall in severity of lung lesions was observed with 50 microg (again both applications). At 100 microg dose, with intramuscular route, a further decrease in the lung bacterial load was shown compared to the 50 microg dose. In contrast, 100 microg LPS, when given intranasally, resulted in a higher bacterial colonization of the lung tissue and higher lung pathology; thus we recommend intramuscular instead of the intranasal route for developing protection against K. pneumoniae-mediated pneumonia with intact LPS-based vaccines.
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Folia Microbiol (Praha). 2003;48(5):699-702 PubMed
Can J Microbiol. 1976 Jan;22(1):29-34 PubMed
Folia Microbiol (Praha). 2003;48(5):665-9 PubMed
Infect Immun. 1990 May;58(5):1421-8 PubMed
Clin Microbiol Rev. 1998 Oct;11(4):589-603 PubMed
Blood. 1998 Apr 1;91(7):2525-35 PubMed
Folia Microbiol (Praha). 2002;47(6):709-16 PubMed
Biochem J. 1956 Feb;62(2):315-23 PubMed
N Engl J Med. 2002 Sep 19;347(12):869-77 PubMed
Am J Pathol. 1991 Jun;138(6):1485-96 PubMed
Am J Physiol. 1996 May;270(5 Pt 1):L836-45 PubMed
Vaccine. 1986 Mar;4(1):15-20 PubMed
Infect Immun. 2000 May;68(5):2402-9 PubMed
Folia Microbiol (Praha). 1996;41(4):373-6 PubMed
Can J Microbiol. 1990 Dec;36(12):885-90 PubMed
J Med Microbiol. 1996 Jan;44(1):44-51 PubMed
Methods Biochem Anal. 1966;14:113-76 PubMed
J Biol Chem. 1975 Apr 25;250(8):2911-19 PubMed
